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Status |
Public on Mar 27, 2024 |
Title |
RNA-seq profiles of DP thymocytes from control and Suv39h1/Suv39h2 double knockout mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
H3K9me3-dependent heterochromatin is critical for the silencing of repeat-rich pericentromeric regions and also has key roles in repressing lineage-inappropriate protein-coding genes for healthy cellular function. Here we investigate the transcriptomic effects of heterochromatin loss in primary immune cells deficient in both Suv39h1 and Suv39h2 (Suv39DKO), the major mammalian histone methyltransferase enzymes which catalyse heterochromatic H3K9me3 deposition.
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Overall design |
Six samples of CD4+CD8+ double positive mouse thymocyte cells were analysed. There were three replicates from Suv39h1+/y Suv39h2+/- (control) mice and three replicates from Suv39h1-/y Suv39h2-/- double knock-out (Suv39DKO) mice. All samples were taken from individual mice.
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Contributor(s) |
Garnham AL, Keenan CR, Chan WF, Johanson TM, Smyth GK, Coughlan HD, Allan RS |
Citation(s) |
38719473 |
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Submission date |
Aug 14, 2020 |
Last update date |
Jun 26, 2024 |
Contact name |
Gordon K Smyth |
E-mail(s) |
smyth@wehi.edu.au
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Phone |
(+61 3) 9345 2326
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Fax |
(+61 3) 9347 0852
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URL |
http://www.wehi.edu.au
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Organization name |
Walter and Eliza Hall Institute of Medical Research
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Department |
Bioinformatics
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Lab |
Smyth
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Street address |
1G Royal Pde
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City |
Parkville |
State/province |
Vic |
ZIP/Postal code |
3052 |
Country |
Australia |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (6)
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GSM4728489 |
Suv39h1, Suv39h2 double knock-out, RNA-Seq replicate 1 |
GSM4728490 |
Suv39h1, Suv39h2 double knock-out, RNA-Seq replicate 2 |
GSM4728491 |
Suv39h1, Suv39h2 double knock-out, RNA-Seq replicate 3 |
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This SubSeries is part of SuperSeries: |
GSE156297 |
RNA-seq, LaminB1 ChIP-seq and in situ Hi-C of double positive thymocytes from control and Suv39h1/Suv39h2 double knockout mice |
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Relations |
BioProject |
PRJNA657287 |
SRA |
SRP277566 |