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Series GSE156296 Query DataSets for GSE156296
Status Public on Mar 27, 2024
Title RNA-seq profiles of DP thymocytes from control and Suv39h1/Suv39h2 double knockout mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary H3K9me3-dependent heterochromatin is critical for the silencing of repeat-rich pericentromeric regions and also has key roles in repressing lineage-inappropriate protein-coding genes for healthy cellular function. Here we investigate the transcriptomic effects of heterochromatin loss in primary immune cells deficient in both Suv39h1 and Suv39h2 (Suv39DKO), the major mammalian histone methyltransferase enzymes which catalyse heterochromatic H3K9me3 deposition.
 
Overall design Six samples of CD4+CD8+ double positive mouse thymocyte cells were analysed. There were three replicates from Suv39h1+/y Suv39h2+/- (control) mice and three replicates from Suv39h1-/y Suv39h2-/- double knock-out (Suv39DKO) mice. All samples were taken from individual mice.
 
Contributor(s) Garnham AL, Keenan CR, Chan WF, Johanson TM, Smyth GK, Coughlan HD, Allan RS
Citation(s) 38719473
Submission date Aug 14, 2020
Last update date Jun 26, 2024
Contact name Gordon K Smyth
E-mail(s) smyth@wehi.edu.au
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
URL http://www.wehi.edu.au
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM4728486 Control, RNA-Seq replicate 1
GSM4728487 Control, RNA-Seq replicate 2
GSM4728488 Control, RNA-Seq replicate 3
This SubSeries is part of SuperSeries:
GSE156297 RNA-seq, LaminB1 ChIP-seq and in situ Hi-C of double positive thymocytes from control and Suv39h1/Suv39h2 double knockout mice
Relations
BioProject PRJNA657287
SRA SRP277566

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156296_Counts_all_samples.txt.gz 610.3 Kb (ftp)(http) TXT
GSE156296_RAW.tar 2.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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