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Status |
Public on Apr 10, 2009 |
Title |
Regulation of T cell gene expression by Halofuginone (HF) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
T cell differentiation to the Th17 effector subset requires stimulation through the T cell and co-stimulatory receptors, together with cytokine stimulation by TGFb and IL-6. The small molecule halofuginone (HF) inhibits Th17 cell development and induces a pattern of stress-regulated gene expression that mimics amino acid starvation. We used global transcript profiling to ask how halofuginone modulates gene expression induced during T cell activaiton and Th17 differentiation
Keywords: T cell activation/ differentiation timecourse
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Overall design |
Purified mouse CD4+ CD25- T cells were activated under Th17 polarizing cytokine conditions and treated with either halofuginone or its inactive derivative, MAZ1310, for 3- or 6-hours.
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Contributor(s) |
Sundrud MS, Benoist C, Feuerer M |
Citation(s) |
19498172 |
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Submission date |
Apr 10, 2009 |
Last update date |
Feb 11, 2019 |
Contact name |
CBDM Lab |
E-mail(s) |
cbdm@hms.harvard.edu
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Phone |
617-432-7747
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Organization name |
Harvard Medical School
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Department |
Microbiology and Immunobiology
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Lab |
CBDM
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Street address |
77 Avenue Louis Pasteur
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (12)
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GSM391214 |
T cells+10nM MAZ1310 for 3 hours-biological replicate 1 |
GSM391215 |
T cells+10nM MAZ1310 for 3 hours-biological replicate 2 |
GSM391216 |
T cells+10nM MAZ1310 for 3 hours-biological replicate 3 |
GSM391217 |
T cells+10nM MAZ1310 for 6 hours-biological replicate 1 |
GSM391218 |
T cells+10nM MAZ1310 for 6 hours-biological replicate 2 |
GSM391219 |
T cells+10nM MAZ1310 for 6 hours-biological replicate 3 |
GSM391220 |
T cells+10nM HF for 3 hours-biological replicate 1 |
GSM391221 |
T cells+10nM HF for 3 hours-biological replicate 2 |
GSM391222 |
T cells+10nM HF for 3 hours-biological replicate 3 |
GSM391223 |
T cells+10nM HF for 6 hours-biological replicate 1 |
GSM391224 |
T cells+10nM HF for 6 hours-biological replicate 2 |
GSM391225 |
T cells+10nM HF for 6 hours-biological replicate 3 |
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Relations |
BioProject |
PRJNA116595 |