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| Status |
Public on Feb 27, 2023 |
| Title |
Single-cell RNA-seq profiling of early murine adrenogonadal development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To generate a gene expression atlas of early adrenogonadal development, we performed single-cell transcriptome profiling using a high throughput droplet-based system (10X Genomics).
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| Overall design |
Posterior trunks (embryonic day (E) 9.0), urogenital ridges (E10.5 and E11.5), gonads and adrenal glands (E12.5) were harvested from XX and XY Nr5a1 Tg mouse embryos (for adrenals glands, only XY embryos were analyzed). Tissues were enzymatically dissociated and single-cell RNA-Seq libraries were prepared using the Chromium Single Cell 3′ v2 Reagent Kit (10x Genomics) according to manufacturer’s protocol. Each condition (organ, sex and developmental stage) was performed in two independent replicates.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/36862551/
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| |
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| Contributor(s) |
Neirijnck Y, Sararols P, Mayère C, Nef S |
| Citation(s) |
36862551 |
| |
| Submission date |
Aug 13, 2020 |
| Last update date |
May 30, 2023 |
| Contact name |
Serge Nef |
| E-mail(s) |
serge.nef@unige.ch
|
| Organization name |
University of Geneva
|
| Department |
Genetic Medicine and Development
|
| Street address |
Rue Michel-Servet 1
|
| City |
Genève |
| ZIP/Postal code |
1206 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (18)
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| GSM4726020 |
E9 posterior trunk XX Rep1 [190401_AGP] |
| GSM4726021 |
E9 posterior trunk XX Rep2 [190408_AGP] |
| GSM4726022 |
E10 urogenital ridge XX Rep1 [170828_AGP] |
|
| Relations |
| BioProject |
PRJNA656996 |
| SRA |
SRP277359 |
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