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Series GSE156175 Query DataSets for GSE156175
Status Public on Apr 15, 2021
Title Single-cell RNA-sequencing of mouse islets exposed to proinflammatory cytokines
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Exposure to proinflammatory cytokines is believed to contribute to pancreatic β-cells during diabetes development. While some cytokine-mediated changes in islet gene expression are known, the heterogeneity of the response is not well-understood. Following 6 hour treatment with interleukin-1 beta (IL-1β) and interferon-gamma (IFN-γ) alone or together, mouse islets were subjected to single-cell RNA-sequencing (scRNA-seq). Inducible nitric oxide synthase (iNOS) mRNA (Nos2), antiviral genes, and immune-associated genes were induced in a subset of β-cells in response to both cytokines, while IL-1β alone activated only antiviral genes. Subsets of α- and δ-cells expressed Nos2 and exhibited similar gene expression changes as β-cells, including induction of antiviral genes and repression of identity genes. Finally, cytokine-responsiveness was inversely correlated with expression of genes encoding heat shock proteins. Our findings show that all endocrine cell types respond to cytokines, IL-1β induces the expression of protective genes in β-cells, and cellular stress gene expression is associated with an inhibition in cytokine signaling.
 
Overall design Four samples of murine islets (no cytokine treatment, IL-1B only, IFNg only, IL-1B and IFNg exposure for 6 hours) were prepared using 10x Genomics Chromium platform
 
Contributor(s) Corbett J, Cui W, Stancill J
Citation(s) 33883217
Submission date Aug 13, 2020
Last update date May 20, 2021
Contact name Weiguo Cui
E-mail(s) wcui04@gmail.com
Phone 414-937-3830
Organization name Blood Center of Wisconisn
Department BRI
Street address 8727 W Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM4726016 scRNAseq_Islets_Untreated
GSM4726017 scRNAseq_Islets_IL1B
GSM4726018 scRNAseq_Islets_IFNg
Relations
BioProject PRJNA656998
SRA SRP277360

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Supplementary file Size Download File type/resource
GSE156175_RAW.tar 97.1 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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