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| Status |
Public on Dec 17, 2020 |
| Title |
scRNAseq in Pten enhancer wild-type or deleted thymocytes, enriched for immature thymocyte stages (CD4-CD3-) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell suspensions of total thymocytes were obtained from Pten enhancer (PE) wild-type or knockout mice. This single-cell suspension was enriched in CD4-CD3- immature thymocyte progenitor cells. CD4-CD3- enriched thymocytes were then mixed 1:1 with single-cell suspensions from total unenriched thymocytes and subsequntly loaded in a 10x Chromium instrument for single-cell RNAseq analyses. Our results revealed Pten levels are signifcantly decreased in CD4-CD8- double negative (DN) thymocytes, CD8+ intermediate single positive (ISP) thymocytes and CD4+CD8+ double positive (DP) thymocytes in PE knockout mice, compared to PE wild-type mice.
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| Overall design |
scRNAseq analysis of mouse thymus in two genotypes and one sample per genotype
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| Contributor(s) |
Tottone L, Lancho O, Loh JW, Singh A, Khiabanian H, Herranz D |
| Citation(s) |
33458694 |
| |
| Submission date |
Aug 11, 2020 |
| Last update date |
Mar 18, 2021 |
| Contact name |
Daniel Herranz |
| E-mail(s) |
dh710@cinj.rutgers.edu
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| Organization name |
Rutgers Cancer Institute of New Jersey
|
| Street address |
195 Little Albany Street
|
| City |
New Brunswick |
| State/province |
New Jersey |
| ZIP/Postal code |
08901 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA656589 |
| SRA |
SRP277104 |
