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Series GSE156070 Query DataSets for GSE156070
Status Public on Dec 17, 2020
Title scRNAseq in Pten enhancer wild-type or deleted thymocytes, enriched for immature thymocyte stages (CD4-CD3-)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Single cell suspensions of total thymocytes were obtained from Pten enhancer (PE) wild-type or knockout mice. This single-cell suspension was enriched in CD4-CD3- immature thymocyte progenitor cells. CD4-CD3- enriched thymocytes were then mixed 1:1 with single-cell suspensions from total unenriched thymocytes and subsequntly loaded in a 10x Chromium instrument for single-cell RNAseq analyses. Our results revealed Pten levels are signifcantly decreased in CD4-CD8- double negative (DN) thymocytes, CD8+ intermediate single positive (ISP) thymocytes and CD4+CD8+ double positive (DP) thymocytes in PE knockout mice, compared to PE wild-type mice.
Overall design scRNAseq analysis of mouse thymus in two genotypes and one sample per genotype
Contributor(s) Tottone L, Lancho O, Loh JW, Singh A, Khiabanian H, Herranz D
Citation(s) 33458694
Submission date Aug 11, 2020
Last update date Mar 18, 2021
Contact name Daniel Herranz
Organization name Rutgers Cancer Institute of New Jersey
Street address 195 Little Albany Street
City New Brunswick
State/province New Jersey
ZIP/Postal code 08901
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM4721907 WT-Thymus
GSM4721908 KO-Thymus
BioProject PRJNA656589
SRA SRP277104

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Supplementary file Size Download File type/resource
GSE156070_RAW.tar 115.0 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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