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Series GSE155874 Query DataSets for GSE155874
Status Public on Dec 14, 2020
Title Modulation of Aubā€“Tdrd interactions elucidates piRNA amplification and germ plasm formation
Organism Drosophila melanogaster
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Aub protein guided by piRNAs ensures genome integrity by cleaving retrotransposons and genome propagation by trapping mRNAs to form the germ plasm that instructs germ cell formation. The amino terminus of Aub (Aub-NT) is rich in arginines (Aub-NTRs), which are symmetrically dimethylated (sDMAs), and interacts with Tudor protein and other Tudor domain containing proteins (Tdrds). Aub-Tdrd interactions play critical roles in suppressing active retrotransposons via piRNA amplification and in germ plasm formation via generation of Aub-Tudor ribonucleoproteins. Here we show that Aub-NTRs are dispensable for primary piRNA biogenesis but essential for piRNA amplification and transposon control and that sDMAs in Aub-NT are essential for germ plasm formation and germ cell specification, but largely dispensable for piRNA amplification and transposon control.
Overall design We examine the importance of Aub N-terminal arginine di-methylation in piRNA pathway and germ plasm formation in Drosophila ovaries
Contributor(s) Vrettos N, Maragkakis M, Alexiou P, Palmieri D, Sgourdou P, Ibrahim F, Kirino Y, Mourelatos Z
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Submission date Aug 07, 2020
Last update date Dec 18, 2020
Contact name Manolis Maragkakis
Organization name University of Pennsylvania
Department Pathology and Laboratory Medicine
Lab Mourelatos Lab
Street address 422 Curie Boulevard
City Philadelphia
State/province PA
ZIP/Postal code 19104-6100
Country USA
Platforms (3)
GPL11203 Illumina Genome Analyzer IIx (Drosophila melanogaster)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
GPL21306 Illumina HiSeq 4000 (Drosophila melanogaster)
Samples (12)
GSM4714979 dme_Ago3_IP_csul_ov_1
GSM4714980 dme_Ago3_IP_RK_ov_1
GSM4714983 dme_Ago3_IP_w_ov_1
BioProject PRJNA655907
SRA SRP276662

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Supplementary file Size Download File type/resource
GSE155874_RAW.tar 60.0 Kb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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