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| Status |
Public on Aug 01, 2021 |
| Title |
Memory-like natural killer cells facilitate effector functions of daratumumab in multiple myeloma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Natural killer (NK) cells support the anti-myeloma activity of daratumumab via antibody-dependent cellular cytotoxicity (ADCC) in multiple myeloma (MM). However, the different roles of heterogeneous NK cell subpopulations have not been elucidated in MM. Here, we delineate memory-like NK cells in the bone marrow (BM) of newly diagnosed MM (NDMM) patients using single-cell RNA sequencing, and further characterize their distinct immunophenotypic features and functions by multicolor flow cytometry. Memory-like NK cells exert robust daratumumab-mediated effector functions ex vivo, including cytokine production and degranulation, compared to conventional NK cells. The composition of memory-like NK cells in BM determines the daratumumab-mediated ex vivo functional activity of BM NK cells in NDMM patients. Unlike conventional NK cells, sorted memory-like NK cells from the BM of NDMM patients exert substantial cytotoxic activity against myeloma cells in the presence of daratumumab. Our findings indicate that memory-like NK cells are an important mediator of daratumumab-dependent effector functions in MM and support direct future efforts to better predict and improve the clinical efficacy of therapeutic antibodies by selectively employing memory-like NK cells.
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| Overall design |
NK cells sorted from 3 MM patients' bone marrow, no replicates
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| Contributor(s) |
Cho H, Kim KH, Shin E, Kim JS |
| Citation(s) |
33602683 |
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| Submission date |
Aug 06, 2020 |
| Last update date |
May 11, 2022 |
| Contact name |
Kyung Hwan Kim |
| E-mail(s) |
knayeah@gmail.com
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| Organization name |
Yonsei University College of Medicine
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| Street address |
50-1 Yonsei-ro
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| City |
Seoul |
| ZIP/Postal code |
03722 |
| Country |
South Korea |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA658903 |
| SRA |
SRP278584 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155795_RAW.tar |
61.4 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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