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Series GSE155543 Query DataSets for GSE155543
Status Public on Mar 23, 2021
Title Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia and mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate a temporal cell atlas of the Drosophila wing disc from two developmental time points. Using these data, we visualized gene expression using a multi-layered model of the wing disc and catalogued ligand-receptor pairs that could mediate signaling between epithelial cells and adult muscle precursors (AMPs). We found that localized expression of the FGF ligands, Thisbe and Pyramus, in the disc epithelium regulates the number and location of the AMPs. In addition, Hedgehog ligand from the epithelium activates a specific transcriptional program within adjacent AMP cells, defined by AMP-specific targets Neurotactin and midline, that is critical for proper formation of direct flight muscles. More generally, our annotated temporal cell atlas provides an organ-wide view of potential cell-cell interactions between epithelial and myogenic cells.
Overall design Cells were dissociated from Drosophila melanogaster (Oregon R strain) wing-imaginal discs, barcoded using the 10x Chromium single-cell RNA sequencing platform, and sequenced on an Illumina NovaSeq. Wing disc samples were collected from two developmental time points, 96 and 120 hours after egg lay. For each time point, two replicates were collected, for a total of 4 sequencing runs.
Contributor(s) Everetts NJ, Worley MI, Yasutomi R, Yosef N, Hariharan IK
Citation(s) 33749594
Submission date Aug 02, 2020
Last update date Mar 24, 2022
Contact name Iswar Krishna Hariharan
Organization name University of California, Berkeley
Department Molecular & Cell Biology
Lab Hariharan Lab
Street address Weill Hall
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
Platforms (1)
GPL25244 Illumina NovaSeq 6000 (Drosophila melanogaster)
Samples (4)
GSM4705964 Dros120H1WingDisc (scRNA-seq)
GSM4705965 Dros120H2WingDisc (scRNA-seq)
GSM4705966 Dros96H1WingDisc (scRNA-seq)
BioProject PRJNA650213
SRA SRP275568

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Supplementary file Size Download File type/resource
GSE155543_RAW.tar 556.0 Mb (http)(custom) TAR (of H5, MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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