 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 23, 2021 |
| Title |
Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia and mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate a temporal cell atlas of the Drosophila wing disc from two developmental time points. Using these data, we visualized gene expression using a multi-layered model of the wing disc and catalogued ligand-receptor pairs that could mediate signaling between epithelial cells and adult muscle precursors (AMPs). We found that localized expression of the FGF ligands, Thisbe and Pyramus, in the disc epithelium regulates the number and location of the AMPs. In addition, Hedgehog ligand from the epithelium activates a specific transcriptional program within adjacent AMP cells, defined by AMP-specific targets Neurotactin and midline, that is critical for proper formation of direct flight muscles. More generally, our annotated temporal cell atlas provides an organ-wide view of potential cell-cell interactions between epithelial and myogenic cells.
|
| |
|
| Overall design |
Cells were dissociated from Drosophila melanogaster (Oregon R strain) wing-imaginal discs, barcoded using the 10x Chromium single-cell RNA sequencing platform, and sequenced on an Illumina NovaSeq. Wing disc samples were collected from two developmental time points, 96 and 120 hours after egg lay. For each time point, two replicates were collected, for a total of 4 sequencing runs.
|
| |
|
| Contributor(s) |
Everetts NJ, Worley MI, Yasutomi R, Yosef N, Hariharan IK |
| Citation(s) |
33749594 |
| |
| Submission date |
Aug 02, 2020 |
| Last update date |
Mar 24, 2022 |
| Contact name |
Iswar Krishna Hariharan |
| E-mail(s) |
ikh@berkeley.edu
|
| Organization name |
University of California, Berkeley
|
| Department |
Molecular & Cell Biology
|
| Lab |
Hariharan Lab
|
| Street address |
Weill Hall
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA650213 |
| SRA |
SRP275568 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155543_RAW.tar |
556.0 Mb |
(http)(custom) |
TAR (of H5, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...