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Series GSE15535 Query DataSets for GSE15535
Status Public on Apr 11, 2009
Title Genome-wide identification of the occupancy of RNA polymerase II (POL II) at L4/young adult stage in C. elegans
Project modENCODE
Organism Caenorhabditis elegans
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We acquied the RNA polymerase II binding profiles in ama-1 transgenic worms. The large subuint of POL II, AMA-1, was C-terminally tagged with GFP, the binding regions were determined using chromatin immunoprecipitation with anti-GFP and anti-polymerase II antibodies followed by illumina high-throughput sequencing (ChIP-seq).

For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
 
Overall design RNA polymerase II binding profiles at L4/young adult stage were acquied with anti-native protein antibody (8WG16) and anti-epitope (GFP) antibody
Web link http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
 
Contributor(s) Zhong M, Niu W
Citation missing Has this study been published? Please login to update or notify GEO.
BioProject PRJNA63461
Submission date Apr 03, 2009
Last update date May 15, 2019
Contact name Flora Vaccarino
Organization name Yale University
Department Child Study Center
Lab Vaccarino
Street address 230 South Frontage Road
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platforms (1)
GPL9309 Illumina Genome Analyzer (Caenorhabditis elegans)
Samples (6)
GSM389308 ama-1 L4/young adult replicate 1 GFP
GSM389309 ama-1 L4/young adult replicate 1 POL II
GSM389310 ama-1 L4/young adult replicate 1 Input
Relations
SRA SRP000863

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15535_ELAND_PEAK_BED_README.txt 2.1 Kb (ftp)(http) TXT
GSE15535_RAW.tar 1000.1 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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