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Series GSE154691 Query DataSets for GSE154691
Status Public on Feb 15, 2021
Title GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary The in vitro reconstitution of human germ-cell development provides a robust framework for clarifying key underlying mechanisms. Here, we explored transcription factors (TFs) that engender the germ-cell fate in their pluripotent precursors. Unexpectedly, SOX17, TFAP2C, and BLIMP1, which act under the BMP signaling and are indispensable for human primordial germ-cell-like cell (hPGCLC) specification, failed to induce hPGCLCs. In contrast, GATA3 or GATA2, immediate BMP effectors, combined with SOX17 and TFAP2C, generated hPGCLCs. GATA3/GATA2 knockouts dose-dependently impaired BMP-induced hPGCLC specification, whereas GATA3/GATA2 expression remained unaffected in SOX17, TFAP2C, or BLIMP1 knockouts. In cynomolgus monkeys, a key model for human development, GATA3, SOX17, and TFAP2C were co-expressed exclusively in early PGCs. Crucially, the TF-induced hPGCLCs acquired a hallmark of bona fide hPGCs to undergo epigenetic reprogramming and mature into oogonia/gonocytes in xenogeneic reconstituted ovaries. By uncovering a TF circuitry driving the germ line program, our study provides a paradigm for TF-based human gametogenesis.
 
Overall design Refer to individual Series
 
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Submission date Jul 19, 2020
Last update date Feb 17, 2021
Contact name Yukihiro Yabuta
E-mail(s) yabyab@anat2.med.kyoto-u.ac.jp
Organization name Kyoto University, Graduate school of medicine
Department Anatomy and Cell Biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (184)
GSM4677257 Overexpression of TFAP2C, BLIMP1, clone_2; iPSC, MS151Y095
GSM4677258 Overexpression of TFAP2C, BLIMP1, clone_2; day2, MS151Y098
GSM4677259 Overexpression of TFAP2C, BLIMP1, clone_2; day4, MS151Y101
This SuperSeries is composed of the following SubSeries:
GSE154688 GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program [RNA-Seq]
GSE154690 GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program [Bisulfite-Seq]
Relations
BioProject PRJNA647202

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154691_RAW.tar 457.1 Mb (http)(custom) TAR (of BIGWIG, TXT)

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