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Series GSE154688 Query DataSets for GSE154688
Status Public on Feb 16, 2021
Title GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program [RNA-Seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The in vitro reconstitution of human germ-cell development provides a robust framework for clarifying key underlying mechanisms. Here, we explored transcription factors (TFs) that engender the germ-cell fate in their pluripotent precursors. Unexpectedly, SOX17, TFAP2C, and BLIMP1, which act under the BMP signaling and are indispensable for human primordial germ-cell-like cell (hPGCLC) specification, failed to induce hPGCLCs. In contrast, GATA3 or GATA2, immediate BMP effectors, combined with SOX17 and TFAP2C, generated hPGCLCs. GATA3/GATA2 knockouts dose-dependently impaired BMP-induced hPGCLC specification, whereas GATA3/GATA2 expression remained unaffected in SOX17, TFAP2C, or BLIMP1 knockouts. In cynomolgus monkeys, a key model for human development, GATA3, SOX17, and TFAP2C were co-expressed exclusively in early PGCs. Crucially, the TF-induced hPGCLCs acquired a hallmark of bona fide hPGCs to undergo epigenetic reprogramming and mature into oogonia/gonocytes in xenogeneic reconstituted ovaries. By uncovering a TF circuitry driving the germ line program, our study provides a paradigm for TF-based human gametogenesis.
 
Overall design 3' RNA-Seq samples of human iPSCs at different time points during PGCLC differentiation with genetic modification
 
Contributor(s) Kojima Y, Yamashiro C, Murase Y, Yabuta Y, Okamoto I, Iwatani C, Tsuchiya H, Nakaya M, Tsukiyama T, Nakamura T, Yamamoto T, Saitou M
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Submission date Jul 19, 2020
Last update date Feb 21, 2021
Contact name Yukihiro Yabuta
E-mail(s) yabyab@anat2.med.kyoto-u.ac.jp
Organization name Kyoto University, Graduate school of medicine
Department Anatomy and Cell Biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (183)
GSM4677257 Overexpression of TFAP2C, BLIMP1, clone_2; iPSC, MS151Y095
GSM4677258 Overexpression of TFAP2C, BLIMP1, clone_2; day2, MS151Y098
GSM4677259 Overexpression of TFAP2C, BLIMP1, clone_2; day4, MS151Y101
This SubSeries is part of SuperSeries:
GSE154691 GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program
Relations
BioProject PRJNA647203
SRA SRP272483

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154688_RAW.tar 81.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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