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Series GSE15464 Query DataSets for GSE15464
Status Public on Sep 30, 2009
Title VEGF induction of HUVEC
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Angiogenesis, the formation of new capillaries by sprouting from preexisting vessels, is mainly induced by VEGF-A. To identify genes which are induced by VEGF-A in endothelial cells, HUVEC were starved and induced by VEGF-A165 for 30, 60 and 150min. RNA of induced and uninduced cells was isolated and subjected to microarray analysis using Affymetrix microarray.
 
Overall design Human umbilical vein endothelial cells were grown in complete EGM-2 medium in dense culture for four days without change of medium and before the stimulation in EGM-2 medium without growth factors over night. The cells were stimulated with 100ng/ml VEGF-A165 for 30, 60 and 150 min. RNA was isolated with Trizol according to the manufacturer's instructions.
 
Contributor(s) Testori J, Bilban M, Hofer E
Citation(s) 19718476
Submission date Mar 31, 2009
Last update date Jan 08, 2019
Contact name Julia Testori
Organization name Medical University Vienna
Department Vascular Biology and Thrombosis Research
Street address Lazarettgasse 19
City Wien
ZIP/Postal code 1090
Country Austria
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (4)
GSM385333 HUVEC_uninduced
GSM385338 HUVEC_VEGF_30min
GSM385350 HUVEC_VEGF_60min
Relations
BioProject PRJNA115901

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15464_RAW.tar 19.1 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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