GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE154452 Query DataSets for GSE154452
Status Public on Jun 25, 2021
Title Cell-to-cell variability in PAX3:FOXO1 expression determines tumorigenic potential in rhabdomyosarcoma
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The rhabdomyosarcoma (RMS) cell pool is phenotypically and functionally heterogeneous. Low passage cell lines established from Myf6Cre,Pax3:Fkhr,p53 mouse RMS and primary human RMS cultures were used to demonstrate marked heterogeneity in PAX3:FOXO1 (P3F) expression at the single-cell level. In mouse Myf6Cre,Pax3:Fkhr,p53 RMS cells, expression of P3F is directed by the Pax3 promoter and coupled to an eYFP fluorescent marker. YFPlow/ P3Flow mouse RMS cells included 87±0.2% G0/ G1 cells, differentially expressed transcripts involved in extracellular matrix interaction and reorganized their actin cytoskeleton to produce a cellular phenotype characterized by more efficient adhesion and migration compared to YFPhigh/ P3Fhigh cells. By contrast, 49±3.2% of YFPhigh/ P3Fhigh cells were in the G2/ M phase of the cell cycle, and higher P3F expression correlated with higher proliferation rates. These differences translated into higher tumor-propagating cell frequencies in limiting dilution analyses and higher clonal activity of YFPlow/ P3Flow compared to YFPhigh/ P3Fhigh cells. Both YFPlow/ P3Flow and YFPhigh/P3Fhigh cells gave rise to mixed clones, consistent with fluctuations in P3F expression at the cellular level over time. Finally, exposure to the anti-tropomyosin compound TR100 disrupted the actin cytoskeleton and reversed more efficient migration and adhesion in YFPlow/ P3Flow RMS cells. We speculate that conversion from one phenotype to another may result in adaptive plasticity and may provide a critical advantage during tumor progression
Overall design We performed ATAC-Seq on YFP high / P3F high and YFP low / P3F low cells isolated from mouse rhabdomyosarcoma cells U23674.
Contributor(s) Ku M, Ke E, Regina C, Hettmer S
Citation(s) 34187933
Submission date Jul 15, 2020
Last update date Jul 06, 2021
Contact name Manching Ku
Organization name Medical Center - University of Freiburg
Department Hematology and Oncology
Street address Mathildenstrasse 1
City Freiburg
ZIP/Postal code 79106
Country Germany
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM4671366 P3F_Low_U23674_ATACSeq
GSM4671367 P3F_high_U23674_ATACSeq
BioProject PRJNA646365
SRA SRP271996

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154452_RAW.tar 380.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap