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| Status |
Public on Sep 29, 2020 |
| Title |
scRNA-seq on C57BL/6J mice retina |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Fresh retinatissues were harvested from 30 day old C57BL/6J mice according to published protocols. 3 retinas were pooled together to form one biological sample. Under a dissecting microscope, spring scissors were used to puncture the eye and remove the cornea, iris, and lens. The remaining eyecup had 4 radial incisions made every 90 degrees, resulting in a flat and open eye cup. The retina was then gently removed using curved tweezers and placed in a 1.5 ml microcentrifuge tube.
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| Overall design |
Fresh retinatissues were harvested from 30 day old C57BL/6J mice according to published protocols. 3 retinas were pooled together to form one biological sample. Under a dissecting microscope, spring scissors were used to puncture the eye and remove the cornea, iris, and lens. The remaining eyecup had 4 radial incisions made every 90 degrees, resulting in a flat and open eye cup. The retina was then gently removed using curved tweezers and placed in a 1.5 ml microcentrifuge tube.
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| Contributor(s) |
Kallestad L, Palczewski K |
| Citation(s) |
32691052 |
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| Submission date |
Jul 05, 2020 |
| Last update date |
Sep 29, 2020 |
| Contact name |
Les Kallestad |
| Organization name |
University of California Irvine
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| Department |
Ophthalmology
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| Lab |
Krzysztof Palczewski
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| Street address |
829 Health Sciences Rd
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| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA644203 |
| SRA |
SRP270068 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE153818_RAW.tar |
19.6 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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