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| Status |
Public on Aug 04, 2021 |
| Title |
Tissue microenvironment shapes distinct sets of machineries to regulate ILC2 properties [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
ILC2s from different tissues manifest distinct properties. To investigate the molecular mechanisms regulating tissue features of ILC2s, we performed single cell RNA-seq on purified ILC2s from 5 different tissues.
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| Overall design |
ILC2s from the bone marrow, large intestine, lung, pancreas and small intestine from 6-week-old male wild-type mice were sorted by flow cytometry. ILC2s from bone marrow, large intestine, lung and pancreas were sorted as Lin(lineage)–ST2+CD25+ cells. And ILC2s from small intestine were sorted as Lin–Thy1intermediateCD45+KLRG1+ cells. Lineage markers were CD3, B220, CD11b, CD11c and FceRI. 30000 ILC2s from 5 tissues of 10 mice were subjected to scRNA-seq and two rounds of sequencing were performed on the each library of ILC2s from every tissue to obtain abundant reads and pooled for subsequent analysis.
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| Contributor(s) |
Qiu J, Qiu J, Zhang J, Shen L, Ji Y |
| Citation(s) |
34349237 |
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| Submission date |
Jul 03, 2020 |
| Last update date |
Aug 06, 2021 |
| Contact name |
Ju Qiu |
| Organization name |
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
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| Street address |
320 Yueyang Road
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE153749 |
Tissue microenvironment shapes distinct sets of machineries to regulate ILC2 properties |
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| Relations |
| BioProject |
PRJNA643922 |
| SRA |
SRP269908 |
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