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| Status |
Public on Mar 26, 2009 |
| Title |
Auxin-induced gene expression in mesocotyl elongation of maize inbred line 3681-4 tolerant to deep-sowing |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
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| Summary |
In order to elucidate the molecular mechanism of auxin-induced mesocotyl elongation, gene expression profiling analyses were performed in a deep-sowing tolerant maize inbred line 3681-4. Gene expression studies combing Affymetrix GeneChip analysis and Real-time PCR were employed to determine the molecular mechanism underlying IAA promotion of maize mesocotyl elongation. Under deep-sowing condition, IAA is transported by auxin transporter-like protein 1 and binds to auxin binding protein ABP20, which results in degradation of Aux/IAA and de-repressing of auxin-inducible genes. Then, transcriptional factor such as MYB, kinase such as LRR, fructose and mannose metabolism and so on are activated. Finally, genes involved in cell wall synthesis and modification are expressed so that mesocotyl elongation of 3681-4 is promoted. Furthermore, gene expression of a key enzyme ACO in ethylene biosynthesis and ethylene receptor ETR2 were up-regulated after the treatment with 10-4 M IAA, which suggested that mesocotyl elongation of 3681-4 inclined to be inhibited when the concentration of applied IAA was increased from 10-4 M to 10-3 M.
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| Overall design |
In two independent experiments, we generate 10-day-old maize mesocotyl-specific gene expression profiles through comparing genome-wide expression patterns of IAA treatment and TIBA (an auxin transportation inhibitor) treatment under 20 cm deep-sowing condition in darkness by using 17,555 Affymetrix maize whole genome array.
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| Contributor(s) |
Guangwu Z, Junjie F, Guoying W, Pan M, Liwen W, Jianhua W |
| Citation missing |
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| Submission date |
Mar 24, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Guoying Wang |
| E-mail(s) |
gywang@caas.net.cn
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| Phone |
86-10-62162927
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Department |
Institute of Crop Sciences
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| Lab |
Maize Molecular Biology
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| Street address |
Zhongguancun Nandajie 12
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| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL4032 |
[Maize] Affymetrix Maize Genome Array |
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| Samples (6)
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| GSM375788 |
Deep-sowing 10d treatment Control, biological rep1 |
| GSM375795 |
Deep-sowing 10d treatment Control, biological rep2 |
| GSM385707 |
Deep-sowing 10d treatment IAA, biological rep1 |
| GSM385708 |
Deep-sowing 10d treatment IAA, biological rep2 |
| GSM385709 |
Deep-sowing 10d treatment TIBA, biological rep1 |
| GSM385710 |
Deep-sowing 10d treatment TIBA, biological rep2 |
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| Relations |
| BioProject |
PRJNA117103 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15371_RAW.tar |
13.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
| Processed data included within Sample table |
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