NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE15371 Query DataSets for GSE15371
Status Public on Mar 26, 2009
Title Auxin-induced gene expression in mesocotyl elongation of maize inbred line 3681-4 tolerant to deep-sowing
Organism Zea mays
Experiment type Expression profiling by array
Summary In order to elucidate the molecular mechanism of auxin-induced mesocotyl elongation, gene expression profiling analyses were performed in a deep-sowing tolerant maize inbred line 3681-4. Gene expression studies combing Affymetrix GeneChip analysis and Real-time PCR were employed to determine the molecular mechanism underlying IAA promotion of maize mesocotyl elongation. Under deep-sowing condition, IAA is transported by auxin transporter-like protein 1 and binds to auxin binding protein ABP20, which results in degradation of Aux/IAA and de-repressing of auxin-inducible genes. Then, transcriptional factor such as MYB, kinase such as LRR, fructose and mannose metabolism and so on are activated. Finally, genes involved in cell wall synthesis and modification are expressed so that mesocotyl elongation of 3681-4 is promoted. Furthermore, gene expression of a key enzyme ACO in ethylene biosynthesis and ethylene receptor ETR2 were up-regulated after the treatment with 10-4 M IAA, which suggested that mesocotyl elongation of 3681-4 inclined to be inhibited when the concentration of applied IAA was increased from 10-4 M to 10-3 M.
 
Overall design In two independent experiments, we generate 10-day-old maize mesocotyl-specific gene expression profiles through comparing genome-wide expression patterns of IAA treatment and TIBA (an auxin transportation inhibitor) treatment under 20 cm deep-sowing condition in darkness by using 17,555 Affymetrix maize whole genome array.
 
Contributor(s) Guangwu Z, Junjie F, Guoying W, Pan M, Liwen W, Jianhua W
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 24, 2009
Last update date Mar 21, 2012
Contact name Guoying Wang
E-mail(s) gywang@caas.net.cn
Phone 86-10-62162927
Organization name Chinese Academy of Agricultural Sciences
Department Institute of Crop Sciences
Lab Maize Molecular Biology
Street address Zhongguancun Nandajie 12
City Beijing
ZIP/Postal code 100081
Country China
 
Platforms (1)
GPL4032 [Maize] Affymetrix Maize Genome Array
Samples (6)
GSM375788 Deep-sowing 10d treatment Control, biological rep1
GSM375795 Deep-sowing 10d treatment Control, biological rep2
GSM385707 Deep-sowing 10d treatment IAA, biological rep1
Relations
BioProject PRJNA117103

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15371_RAW.tar 13.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap