|
| Status |
Public on Jul 01, 2020 |
| Title |
m6A directs the demethylation of histone H3K9me2 co-transcriptionall |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
Here we show the repressive H3K9me2 mark is specifically removed by the induction of m6A-modified transcripts. We demonstrate that the methyltransferase METTL3/METTL14 regulates H3K9me2 modification. We observed a genome-wide correlation between m6A and occupancy by the H3K9me2 demethylase KDM3B, and we found m6A reader YTHDC1 physically interacts and recruits KDM3B to m6A-associated chromatin regions, promoting H3K9me2 demethylation and gene expression. This study establishes a direct link between m6A and dynamic chromatin modification and provides mechanistic insight into the co-transcriptional interplay between RNA modification and histone modifications.
|
| |
|
| Overall design |
Examination of m6A methylation and H3K9me2 histone modification
|
| |
|
| Contributor(s) |
Xia L, Xiao S |
| Citation(s) |
32778823 |
| |
| Submission date |
Jul 01, 2020 |
| Last update date |
Aug 19, 2020 |
| Contact name |
linjian xia |
| E-mail(s) |
xia17720507672@gmail.com
|
| Phone |
17720507672
|
| Organization name |
wuhan University
|
| Street address |
wuhan
|
| City |
wuhan |
| ZIP/Postal code |
420000 |
| Country |
China |
| |
|
| Platforms (3) |
| GPL21273 |
HiSeq X Ten (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (56)
|
|
| Relations |
| BioProject |
PRJNA643545 |
| SRA |
SRP269587 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE153651_D395A-m6A_mES.bed.gz |
789.1 Kb |
(ftp)(http) |
BED |
| GSE153651_H3K9me2_D395A_IP_1.bw |
176.6 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_D395A_IP_2.bw |
167.9 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_D395A_Input.bw |
161.2 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_WT_IP_1.bw |
161.9 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_WT_IP_2.bw |
158.0 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_WT_Input.bw |
162.8 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shALKBH5_IP1.bw |
170.5 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shALKBH5_IP2.bw |
177.2 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shALKBH5_Input1.bw |
181.6 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shALKBH5_Input2.bw |
181.2 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shFTO_IP1.bw |
178.4 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shFTO_IP2.bw |
175.6 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shFTO_Input1.bw |
180.4 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shFTO_Input2.bw |
169.7 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shGFP_IP.bw |
182.5 Mb |
(ftp)(http) |
BW |
| GSE153651_H3K9me2_shGFP_Input.bw |
162.0 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_D395A_IP.bw |
100.3 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_D395A_IP2.bw |
84.9 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_D395A_Input.bw |
134.0 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_WT_IP.bw |
53.8 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_WT_IP2.bw |
54.4 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_WT_Input.bw |
132.1 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_shGFP_IP.bw |
52.2 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_shGFP_Input.bw |
169.6 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_shYTHDC1_IP1.bw |
121.2 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_shYTHDC1_IP2.bw |
137.4 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_shYTHDC1_Input1.bw |
156.7 Mb |
(ftp)(http) |
BW |
| GSE153651_KDM3B_shYTHDC1_Input2.bw |
170.1 Mb |
(ftp)(http) |
BW |
| GSE153651_METTL3_IP_1.bw |
138.0 Mb |
(ftp)(http) |
BW |
| GSE153651_METTL3_IP_2.bw |
132.1 Mb |
(ftp)(http) |
BW |
| GSE153651_METTL3_IP_3.bw |
132.7 Mb |
(ftp)(http) |
BW |
| GSE153651_METTL3_Input.bw |
170.7 Mb |
(ftp)(http) |
BW |
| GSE153651_Pol2_IP.bw |
91.8 Mb |
(ftp)(http) |
BW |
| GSE153651_Pol2_Input.bw |
127.5 Mb |
(ftp)(http) |
BW |
| GSE153651_RNA-seq.count.txt.gz |
3.7 Mb |
(ftp)(http) |
TXT |
| GSE153651_WT-m6A_mES.bed.gz |
1.3 Mb |
(ftp)(http) |
BED |
| GSE153651_shALKBH5-m6A_HEK293T_Rep1.bed.gz |
417.4 Kb |
(ftp)(http) |
BED |
| GSE153651_shALKBH5-m6A_HEK293T_Rep2.bed.gz |
526.8 Kb |
(ftp)(http) |
BED |
| GSE153651_shFTO-m6A_HEK293T_Rep1.bed.gz |
412.3 Kb |
(ftp)(http) |
BED |
| GSE153651_shFTO-m6A_HEK293T_Rep2.bed.gz |
571.1 Kb |
(ftp)(http) |
BED |
| GSE153651_shGFP-m6A_HEK293T_Rep1.bed.gz |
498.1 Kb |
(ftp)(http) |
BED |
| GSE153651_shGFP-m6A_HEK293T_Rep2.bed.gz |
487.0 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
Less...
More...