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| Status |
Public on Oct 30, 2020 |
| Title |
The next-generation RNA and ATAC sequencing of MLL-ENL transformed cell lines reveals that C/EBP transcription factors coordinate the expression of the MLL-ENL/Hoxa target genes [ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the application of next-generation RNA and ATAC sequencing technology for high-throughput profiling of MLL-ENL transformed cell lines. According to our results, the C/EBP transcription factors coordinate the expression of the MLL-ENL/Hoxa target genes. Genetic removal of both Cebpa and Cebpb revealed a surrogate function of C/EBPε for myeloid MLL-ENL transformation.
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| Overall design |
mRNA profiles of MLL-ENL transformed cell lines: wild-type, Cebpa/Cebpb dKO clones, and Cebpa/Cebpb dKO clones expressing exogenous rat C/EBP LAP* isoform.
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| |
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| Contributor(s) |
Wesolowski R, Zimmermann K, Borodina TA, Mildner A, Dörr D, Hofstätter M, Kowentz-Leutz E, Leutz A |
| Citation(s) |
33144337 |
| Submission date |
Jul 01, 2020 |
| Last update date |
Dec 01, 2020 |
| Contact name |
Achim Leutz |
| E-mail(s) |
aleutz@mdc-berlin.de
|
| Organization name |
MDC Berlin
|
| Department |
Cancer Research
|
| Lab |
Cell Differentiation and Tumorigenesis
|
| Street address |
Robert-Rössle-Str. 10
|
| City |
Berlin |
| ZIP/Postal code |
13092 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE153624 |
The next-generation RNA and ATAC sequencing of MLL-ENL transformed cell lines reveals that C/EBP transcription factors coordinate the expression of the MLL-ENL/Hoxa target genes |
|
| Relations |
| BioProject |
PRJNA643501 |
| SRA |
SRP269524 |
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