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| Status |
Public on Sep 08, 2020 |
| Title |
Transcriptional profile of B cells isolated from neonatal and adult hearts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have identified four distinct B cell subgroups within the naïve murine heart, whose relative expression changed markedly throughout development, including CD19+CD11b+CD5+, CD19+CD11b+CD5-, CD19+CD11b-CD21+CD23+, and CD19+CD11b-CD21-CD23- B cells. In order to gain further insight into the identity of the various myocardial B cells subsets, we performed single cell RNA sequencing (scRNAseq) of neonatal (2 weeks) and adult (8 weeks) myocardial B cells. We combined 10X single cell gene expression analysis with immunostaining using TotalSeq antibodies against CD11b, CD23, and CD21.
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| Overall design |
We performed transcriptional profiling of cardiac B cells isolated from 6 neonatal (2 weeks old) and 6 adult (8 weeks old) wild-type mice. We used the Chromium Single Cell 3′ v3 Chromium instrument (from 10x Genomics) to perform single cell transcriptional profiling analyses.
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| Contributor(s) |
Rocha-Resende C, Yang W, Adamo L, Mann DL |
| Citation(s) |
32663200 |
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| Submission date |
Jun 30, 2020 |
| Last update date |
Sep 08, 2020 |
| Contact name |
Luigi Adamo |
| E-mail(s) |
ladamo@wustl.edu
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| Phone |
314-273-7987
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| Organization name |
Washington University In St Louis
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| Department |
Internal Medicine - Cardiovascular Division
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| Street address |
660 South Euclid Ave
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| City |
St Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA643253 |
| SRA |
SRP269403 |
