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Series GSE153469 Query DataSets for GSE153469
Status Public on Dec 16, 2020
Title Kisspeptin influences the reproductive axis and circulating levels of microRNAs in Senegalese sole
Organism Solea senegalensis
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The kiss system is known to regulate puberty and to be involved in the onset of reproduction, acting upstream of the Brain-Pituitary-Gonad axis in gonadotrophin releasing hormone neurons and stimulating secretion of GnRH. Kisspeptin has been proven to be a novel therapeutic for fertility disorders in humans. In fish, positive results have also observed the prospective of this hormone to induce gamete development. During the last two decades, support has been obtained describing the key role of microRNAs (miRNA) in regulating gene expression at the post-transcriptional level and they have been recommended as relevant biomarkers in reproductive studies. Senegalese sole is a marine flatfish species with high importance in the European aquaculture industry, due to its good growth rate and high market value. However, this species presents a bottleneck in terms of reproduction, which does not allow to close the cycle in captive conditions. Hence, this study aimed to observe the effect of a novel hormonal treatment using kisspeptin in miRNA expression related to reproductive performance in cultured Senegalese sole breeders. For this purpose, Senegalese sole F1 broodstock were held at CCMAR facilities in 4 tanks of 3m3 (n = 12/per tank, sex ratio 1:1) under natural photoperiod and temperature conditions. During the reproductive season, the animals from each 2 of the tanks were treated either with SSkiss2_v2 decapeptide in a single intramuscular injection, or with a PBS injection for control groups. After the treatment (2 and 4 days), all fish were anesthetized and sampled for blood collection. Afterwards, plasma was obtained to determine several parameters related to reproductive physiology (Fsh, Lh and sexual esteroids (T, 11-KT and E2)) and miRNAs regulation. After observing the high implication of this treatment in the gonadotrofins, above all in Fsh and sexual esteroids, above all in T, the RNA was extracted from blood plasma using miRNeasy Serum/Plasma Kit (Qiagen, Germany) and libraries were prepared using NEXTflex Small RNA-Seq kit v3 (Bioo Scientific, USA). Small RNA sequencing (sRNA-seq) (NextSeq 500) was performed on sole treated with kisspeptin (n = 16) and non-treated control fish (n = 14). A total of 1,467 unique miRNAs were expressed in Senegalese sole plasma. A multidimensional scaling (MDS) plot showed no clear clustering of the samples with the kisspeptin treatment. However, the differential expression analysis revealed one miRNA, miR-1-3p, to be highly up-regulated in both males and females treated with kisspeptin compared to control sole. This miRNA is linked with myogenesis and angiogenesis, and skeletal muscle development in fish. Three isomirs (let 7e, miR-199a-3p, miR-100-5p) were differentially expressed between females treated and control fish. MicroRNA let-7e is an important regulator of endothelial function and inflammation, related to testosterone pathway release; miR-199a-3p plays an important role in inflammation and women fertility and miR-100-5p might be implicated in the regulation of cell growth and oocytes maturation. In conclusion, kisspeptin treatment may affect gonad maturation in females and muscle growth in males. Our data support the possibility of using circulating miRNAs as less invasive physiological biomarkers in fish reproduction.
 
Overall design Examination of the application of a hormonal treatment in blood samples of Senegalese sole species at several levels (endocrine, physiological and circulating miRNAs)
 
Contributor(s) Oliveira C, Fatsini E, Fernández I, Anjos C, Chauvigné F, Cerdà J, Mjelle R, Fernandes J, Cabrita E
Citation(s) 33260781
Submission date Jun 29, 2020
Last update date Dec 17, 2020
Contact name Elvira Fatsini
E-mail(s) effernandez@ualg.pt
Organization name UALG
Street address Universidade do Algarve
City Faro
ZIP/Postal code 8005-139
Country Portugal
 
Platforms (1)
GPL28783 Illumina NextSeq 500 (Solea senegalensis)
Samples (30)
GSM4644641 A1-1_S1
GSM4644642 A1-2_S2
GSM4644643 A1-3_S3
Relations
BioProject PRJNA642787
SRA SRP269187

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE153469_Processed_data.mat.csv.gz 35.3 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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