NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE15342 Query DataSets for GSE15342
Status Public on Apr 03, 2009
Title Microarray analysis of gene expression in the skin of tick-naïve and tick-infested Holstein-Friesian and Brahman cattle
Platform organism Bos taurus
Sample organisms Bos taurus; Bos indicus
Experiment type Expression profiling by array
Summary This experiment was undertaken to document changes in gene expression in the skin of tick-resistant Brahman (Bos indicus) and tick-susceptible Holstein-Friesian (Bos taurus) cattle prior to, and following, infestation with the cattle tick Rhipicephalus (Boophilus) microplus

Keywords: Disease state analysis
 
Overall design RNA was extracted from skin samples of tick-naïve cattle (animals with no previous R.microplus exposure) and tick-infested cattle after a period of successive, heavy infestations with R. microplus. Skin samples taken from tick-infested animals were taken at sites where tick larvae (approximately 24 h old) were attached to the skin sample. Skin samples were of 8 mm diameter and full skin thickness (approximately 10 mm). RNA samples from 12 animals (3 tick-naive Holstein-Friesian, 3 tick-naive Brahman, 3 tick-infested Holstein-Friesian and 3 tick-infested Brahman) were processed and hybridised to individual slides.
 
Contributor(s) Piper EK, Jonsson NN, Jackson LA, Lew AE, Gondro C
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 23, 2009
Last update date Dec 17, 2012
Contact name Emily Kate Piper
E-mail e.piper@uq.edu.au
Organization name University of Queensland
Department School of Veterinary Science
Street address Seddon Buildings (Bldg 82)
City Brisbane
ZIP/Postal code 4072
Country Australia
 
Platforms (1)
GPL2112 [Bovine] Affymetrix Bovine Genome Array
Samples (12)
GSM385316 Naïve Holstein 1
GSM385317 Naïve Holstein 2
GSM385318 Naïve Holstein 3
Relations
BioProject PRJNA116571

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15342_RAW.tar 40.1 Mb (http)(custom) TAR (of CEL)
GSE15342_supplementary_analysis_files.zip 2.8 Mb (ftp)(http) ZIP
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap