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Series GSE153319 Query DataSets for GSE153319
Status Public on Feb 16, 2021
Title Single cell transcriptomc analysis of CD34+ cells from primary myelofibrosis patient
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We performed single cell RNA sequencing by means of 10X Genomics platform of CD34+ hematopoietic stem/progenitor cells (HSPCs) from primary myelofibrosis (PMF). Cells were isolated through immunomagnetic selection from frozen PBMCs coming from one patient with PMF who evolved to Acute Myeloid Leukemia (AML). Three timepoints were considered: chronic phase (Time1), after 8 months of Ruxolitinib treatment (Time2) and after 11 months of Ruxolitinib treatment at AML diagnosis (Time3). The main purpose of this project was to reconstruct the composition of the neoplastic clone in terms of HSPCs subpopulations at different time-points during disease evolution and to study changes in gene expression within each cell cluster over time.
Overall design This dataset include 3 samples from the same patient affected by PMF. The first sample correspond to the chronic phase of the disease. The second one is taken 8 month after Ruxolitinib treatment initiation. The third sample was collected 11 month after Ruxolitinib treatment initiation at the time of AML diagnosis.
Contributor(s) Parenti S, Carretta C, Mallia S, Rontauroli S, Genovese E, Chiereghin C, Peano C, Bianchi E, Fantini S, Sartini S, Tavernari L, Romano O, Bicciato S, Tagliafico E, Della Porta M, Manfredini R
Citation(s) 33542466
Submission date Jun 26, 2020
Last update date Feb 16, 2021
Contact name Rossella Manfredini
Phone +390592058065
Organization name Centre for Regenerative Medicine
Department Life Sciences
Street address Via Gottardi 100
City Modena
ZIP/Postal code 41100
Country Italy
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (3)
GSM4640392 Chronic phase
GSM4640393 Treatment
GSM4640394 Leukemic evolution
BioProject PRJNA642126
SRA SRP268989

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Supplementary file Size Download File type/resource
GSE153319_RAW.tar 115.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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