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Series GSE15295 Query DataSets for GSE15295
Status Public on May 13, 2009
Title Mercury toxicity in barley roots
Organism Hordeum vulgare
Experiment type Expression profiling by array
Summary The effects of mercury (HgCl2) on barley (Hordeum vulgare L.) growth, physiological traits and gene expression profiles were studied. The shoot to root ratio was decreased in the two levels of HgCl2 (500 and 1000 μM) assayed, which was related primarily with decreases in shoot dry weight. Moreover stomatal conductance was limited and leaf carbon isotope discrimination decreased. Therefore water uptake limitations seem to be an important component of barley responses to HgCl2. Evidences for decreased stomatal conductance and water uptake limitations were further confirmed by the over expression of ABA related transcripts and down regulation of an aquaporin in roots. Root dry weight was only affected at 1000 μM HgCl2 and root browning was observed, while several transcripts for lignin biosynthesis were up regulated in HgCl2. Microarray analysis further revealed that growth inhibition in HgCl2 was related to increased expression of genes participating in ethylene biosynthesis and down regulation of several genes participating in DNA synthesis, chromatin structure and cell division, cell wall degradation and modification, oxidative pentose phosphate cycle and nitrogen metabolism pathway. Genes involved in detoxification and defence mechanisms were up regulated including several cytochrome P450s, glucosyltransferases and glutathione-s-transferases and amino acid metabolism participatory genes. It is concluded that barley plants survive in the presence of HgCl2 through several mechanisms that include water uptake limitations, shoot and root growth regulation, increased expression of genes involved in the biosynthesis of several plant protection secondary metabolites and finally through detoxification.
Overall design Six samples were analysed including 3 biological replicates of mercury exposed roots and 3 controls (no mercury added to the growing solution)
Contributor(s) Lopes MS, Iglesia-Turino S, Febrero A, Cabrera-Bosquet L, Bort J, Araus JL
Citation(s) 23925371
Submission date Mar 19, 2009
Last update date Mar 04, 2014
Contact name Marta Silva Lopes
Fax 525558047558
Organization name cimmyt
Department wheat physiology
Street address km 45, carretera Mexico-Veracruz, El Batan, Texcoco
City Texcoco
State/province Mexico
ZIP/Postal code 56130
Country Mexico
Platforms (1)
GPL1340 [Barley1] Affymetrix Barley Genome Array
Samples (6)
GSM382232 Root_Hg1
GSM382233 Root_Hg2
GSM382234 Root_Hg3
BioProject PRJNA116587

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Supplementary file Size Download File type/resource
GSE15295_RAW.tar 13.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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