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| Status |
Public on Oct 28, 2020 |
| Title |
Targeted single cell RNA sequencing of T cells infiltrated in mouse gastric cancer. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
YTN2 and YTN16 cells are chemically induced gastric cancer cell lines. YTN2 is spontaneously regressed in C57BL/6 mice in CD8+ T cell-dependent manner, while YTN16 grows progressively. To investigate tumor infiltrating T cells, we performed single-cell RNA-Seq of CD4+ and CD8+ T cells in YTN2 and YTN16 gastric tumor-bearing mice for 44 T cell-related genes (Supplementary Table 1).
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| Overall design |
Mice were inoculated with 5 ×106 YTN2 or YTN16 on day 0. Tumors were harvested on day 7 and CD4+ and CD8+ T cells were sorted. Single-cell RNA-Seq was performed using vertical flow array chips (Shirai et al, Scientific Reports, 6, srep36014, 2016).
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| Contributor(s) |
Nagaoka K, Shirai M, Kakimi K |
| Citation(s) |
33093158 |
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| Submission date |
Jun 20, 2020 |
| Last update date |
Oct 28, 2020 |
| Contact name |
Koji Nagaoka |
| Organization name |
Kindai University Faculty of Medicine
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| Department |
Department of Immunology
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| Street address |
377-2 Ohno-Higashi, Osaka-Sayama
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| City |
Osaka |
| ZIP/Postal code |
5898511 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (1) |
| GSM4629065 |
Single-cell RNA-Seq of Tumor-infiltrating T cells |
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| Relations |
| BioProject |
PRJNA640802 |
| SRA |
SRP268139 |
