|
| Status |
Public on Jan 04, 2021 |
| Title |
TT-TL-seq reveals transcriptional profiles that accompany DoG induction after hyperosmotic stress. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
TT-TimeLapse-seq was used to investigate the nascent transcription profiles that accompany the induction of downstream-of-gene (DoG) containing transcripts in human cell lines. Data from HEK-293T cells exposed to hyperosmotic stress and cells transfected with siRNAs against Integrator subunit 11 are included.
|
| |
|
| Overall design |
Samples were treated with 80 mM KCl for 1 hour or with siRNAs for 48 hours. During the last 5 minutes of treatment, nascent RNAs were labeled with 1 mM s4U. After RNA extractions using TRIzol, 4% RNA from Drosophila S2 cells was added to each sample. TT-TL-seq preparations were done as described in Schofield et al. 2018.
|
| |
|
| Contributor(s) |
Rosa-Mercado NA, Zimmer JT, Apostolidi M, Rinehart J, Simon MD, Steitz JA |
| Citation(s) |
33400923 |
| |
| Submission date |
Jun 08, 2020 |
| Last update date |
Apr 05, 2021 |
| Contact name |
Nicolle A. Rosa-Mercado |
| E-mail(s) |
nicolle.rosa-mercado@yale.edu
|
| Organization name |
Yale University
|
| Department |
Molecular Biophysics and Biochemistry
|
| Lab |
Joan A. Steitz
|
| Street address |
295 Congress Avenue
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE152063 |
Hyperosmotic stress induces downstream-of-gene transcription and alters the RNA Polymerase II interactome despite widespread transcriptional repression |
|
| Relations |
| BioProject |
PRJNA638166 |
| SRA |
SRP266497 |
Less...
More...