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| Status |
Public on Jun 23, 2020 |
| Title |
A single cell transcriptional atlas of early synovial joint development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Synovial joint development begins with the formation of the interzone, a region of condensed mesenchymal cells at the site of the prospective joint. Recently, lineage tracing strategies have revealed that Gdf5-lineage cells native to and from outside the interzone contribute to most, if not all, of the major joint components. However, there is limited knowledge of the specific transcriptional and signaling programs that regulate interzone formation and fate diversification of synovial joint constituents. To address this, we have performed single cell RNA-Seq analysis of 7,329 synovial joint progenitor cells from the developing murine knee joint from E12.5 to E15.5. By using a combination of computational analytics, in situ hybridization, and in vitro characterization of prospectively isolated populations, we have identified the transcriptional profiles of the major developmental paths for joint progenitors. Our freely available single cell transcriptional atlas will serve as a resource for the community to uncover transcriptional programs and cell interactions that regulate synovial joint development.
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| Overall design |
We sampled Gdf5-lineage cells of the murine knee at embryonic time points e12.5, e13.5, e14.5, and e15.5 and subjected them to single cell RNAseq analysis via 10x.
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| Contributor(s) |
Bian Q, Cahan P |
| Citation(s) |
32580935 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM124725 |
From intra to intercellular regulatory networks that define cell type identity |
JOHNS HOPKINS UNIVERSITY |
Patrick Cahan |
|
| Submission date |
Jun 08, 2020 |
| Last update date |
Sep 22, 2020 |
| Contact name |
Patrick Cahan |
| E-mail(s) |
patrick.cahan@jhmi.edu
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| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
ICE and Biomedical Engineering
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| Lab |
Cahan Lab
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| Street address |
733 North Broadway, MRB 653
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA638016 |
| SRA |
SRP266397 |
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