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Series GSE151928 Query DataSets for GSE151928
Status Public on Oct 21, 2020
Title Bronchoalveolar lavage of healthy human adults
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Rationale: Airspace macrophages are the most abundant cell in airspaces and are viewed as a homogeneous population during health. Single cell RNA sequencing allows for examination of transcriptional heterogeneity between cells and between individuals. Understanding the conserved repertoire of airspace leukocytes during health is essential to understanding cellular programing during disease. Objective: We sought to determine the transcriptional heterogeneity of human bronchoalveolar lavage cells in healthy adults. Methods: Ten healthy subjects underwent bronchoscopy. Cells obtained from lavage fluid were subjected to single cell RNA sequencing. Unique cell populations and putative functions were identified. Transcriptional profiles were compared across individuals. Measurements and Main Results: Based on transcriptional profiling we identify highly conserved macrophage, monocyte-like, lymphocyte, dendritic cell, and cycling cell populations. We define two unique subgroups of resident airspace macrophages - one defined by a pro-inflammatory profile and one by metallothionein gene expression. We identify distinct subsets of monocyte-like cells and directly compared them to peripheral blood mononuclear cells. Finally, we compare global macrophage and monocyte programing between male and female subjects. Conclusions: Healthy human airspaces contain multiple populations of leukocytes that are highly conserved between individuals and between the sexes. Resident macrophages comprise the largest population and include novel subsets defined by inflammatory and metal-binding gene signatures. Monocyte-like cells within the airspaces are transcriptionally distinct from circulating blood cells and include a rare population defined by expression of cell-matrix interaction genes. This study is the first to define airspace immune cell heterogeneity and identifies three previously unrecognized myeloid cell subsets.
 
Overall design Ten bronchoalveolar lavage samples from ten healthy adults were included.
 
Contributor(s) Mould KJ, Moore CM, Hott SA, McCubbrey AL, Griesmer C, Henson PM, Janssen WJ
Citation(s) 33079572
Submission date Jun 06, 2020
Last update date Oct 21, 2020
Contact name Camille Moore
E-mail(s) mooreca@njhealth.org
Organization name National Jewish Health
Street address 1400 Jackson St.
City Denver
State/province CO
ZIP/Postal code 80206
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (10)
GSM4593888 Subject 1
GSM4593889 Subject 2
GSM4593890 Subject 3
Relations
BioProject PRJNA637757
SRA SRP266231

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Supplementary file Size Download File type/resource
GSE151928_RAW.tar 195.3 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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