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Series GSE151828 Query DataSets for GSE151828
Status Public on Jun 04, 2021
Title Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Other
Summary CSR-1 is an essential Argonaute protein that binds to a subclass of 22G-RNAs targeting most germline-expressed genes. Here we show that the two isoforms of CSR-1 have distinct expression patterns; CSR-1B is ubiquitously expressed throughout the germline and during all stages of development while CSR-1A expression is restricted to germ cells undergoing spermatogenesis. Furthermore, CSR-1A associates preferentially with 22G-RNAs mapping to spermatogenesis-specific genes whereas CSR-1B-bound small RNAs map predominantly to oogenesis-specific genes. Interestingly, the exon unique to CSR-1A contains multiple dimethylarginine modifications, which are necessary for the preferential binding of CSR-1A to spermatogenesis-specific 22G-RNAs. Thus, we have discovered a regulatory mechanism for C. elegans Argonaute proteins that allows for specificity of small RNA binding between similar Argonaute proteins with overlapping temporal and spatial localization.
 
Overall design 20 small-RNA seq libraries from immunoprecipitated samples (10 input, 10 IP) in either duplicate or triplicate, one small-RNA seq library from whole worm lysate in duplicate, and three mRNA-seq libraries in triplicate were generated and analyzed.
 
Contributor(s) Phillips CM, Nguyen DH
Citation(s) 34244496
Submission date Jun 04, 2020
Last update date Jul 27, 2021
Contact name Carolyn Marie Phillips
E-mail(s) cphil@usc.edu
Organization name University of Southern California
Department Biological Sciences
Lab Phillips
Street address 1050 Childs Way, RRI 201
City Los Angeles
State/province CA
ZIP/Postal code 90089
Country USA
 
Platforms (2)
GPL13657 Illumina HiSeq 2000 (Caenorhabditis elegans)
GPL19757 Illumina NextSeq 500 (Caenorhabditis elegans)
Samples (60)
GSM4591928 CSR-1A (L4) - input, replicate 2
GSM4591929 CSR-1A (L4) - input, replicate 3
GSM4591930 CSR-1A (L4) - IP, replicate 1
Relations
BioProject PRJNA637379
SRA SRP266192

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151828_RAW.tar 83.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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