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| Status |
Public on Jun 04, 2020 |
| Title |
RNA-sequencing study of global gene expression in haploid yeast Saccharomyces cerevisiae mating type MATa treated with different doses of alpha-pheromone |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Yeast cells were treated with different doses of pheromone in order to obtain dose-response profiles for induced genes. Specifically, dose-dependencies of transcriptional feedback regulators of the pheromone-response pathway were quantified.
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| Overall design |
Yeast cells were grown in minal medium with glucose and treated with pheromone at mid-exponential phase. Samples were collected after 60 minutes of incubation with pheromone. Two biological replicates were performed, each with 9, partially overlapping, doses of pheromone. A sample with mock-treatment, i.e., no addition of pheromone, served as a reference for non-treated cells in each experiment.
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| Contributor(s) |
Anders A, Ghosh B, Glatter T, Sourjik V |
| Citation(s) |
32661402 |
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| Submission date |
Jun 03, 2020 |
| Last update date |
Jul 27, 2020 |
| Contact name |
Victor Sourjik |
| E-mail(s) |
victor.sourjik@mpi-marburg.mpg.de
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| Organization name |
Max Planc Institute for Terrestrial Microbiology
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| Department |
Systems and Synthetic Microbiology
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| Street address |
Karl-von-Frisch-Straße 10
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| City |
Marburg |
| State/province |
Hessen |
| ZIP/Postal code |
35043 |
| Country |
Germany |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (18)
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| GSM4589224 |
10 nM, replicate 1 |
| GSM4589225 |
15 nM, replicate 1 |
| GSM4589226 |
20 nM, replicate 1 |
| GSM4589227 |
50 nM, replicate 1 |
| GSM4589228 |
100 nM, replicate 1 |
| GSM4589229 |
500 nM, replicate 1 |
| GSM4589230 |
0 nM, replicate 2 |
| GSM4589231 |
0.05 nm, replicate 2 |
| GSM4589232 |
0.25 nM, replicate 2 |
| GSM4589233 |
1 nM, replicate 2 |
| GSM4589234 |
5 nM, replicate 2 |
| GSM4589235 |
10 nM, replicate 2 |
| GSM4589236 |
20 nM, replicate 2 |
| GSM4589237 |
50 nM, replicate 2 |
| GSM4589238 |
500 nM, replicate 2 |
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| Relations |
| BioProject |
PRJNA637058 |
| SRA |
SRP265824 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE151729_RPKM_YEAST_pheromone_1.txt.gz |
521.1 Kb |
(ftp)(http) |
TXT |
| GSE151729_RPKM_YEAST_pheromone_2.txt.gz |
526.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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