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Series GSE151715 Query DataSets for GSE151715
Status Public on Apr 15, 2021
Title Protease-mediated processing of Argonaute proteins controls small RNA association [smallRNA-Seq]
Organism Caenorhabditis elegans
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Small RNA pathways defend the germlines of animals against selfish genetic elements and help to maintain genomic integrity. At the same time, their activity needs to be well-controlled to prevent silencing of ‘self’ genes. Here, we reveal a proteolytic mechanism that controls endogenous small interfering (22G) RNA activity in the Caenorhabditis elegans germline to protect genome integrity and maintain fertility. We find that WAGO-1 and WAGO-3 Argonaute proteins are produced as pro-proteins that are matured through proteolytic processing of their unusually proline-rich N-termini. In the absence of DPF-3, a P-granule-localized N-terminal dipeptidase orthologous to mammalian DPP8/9, processing fails, causing a change of identity of 22G RNAs bound to these WAGO proteins. Accumulation of repeat- and transposon-derived transcripts, DNA damage and sterility ensue. We propose that DPF-3 acts as a licensing factor for 22G RNA activity.
Overall design Wild type worms.9 Replicates; dpf-3 null worms. 3 replicates; DPF-3 S784A mutant worms. 3 Replicates; mut-2(ne3370). 3 replicates; mut-7(ne4255). 3 replicates
Contributor(s) Grosshans H, Gudipatti RK, Gypas F
Citation(s) 33852894
Submission date Jun 03, 2020
Last update date Jul 15, 2021
Contact name Foivos Gypas
Organization name FMI
Lab Grosshans
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
Samples (21)
GSM4589013 wild_type_A
GSM4589014 wild_type_B
GSM4589015 wild_type_C
This SubSeries is part of SuperSeries:
GSE151717 Protease-mediated processing of Argonaute proteins controls small RNA association
BioProject PRJNA637042
SRA SRP265813

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Supplementary file Size Download File type/resource
GSE151715_small_RNA_seq_15_C_counts.tsv.gz 759.4 Kb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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