|
| Status |
Public on Apr 15, 2021 |
| Title |
Protease-mediated processing of Argonaute proteins controls small RNA association [smallRNA-Seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Small RNA pathways defend the germlines of animals against selfish genetic elements and help to maintain genomic integrity. At the same time, their activity needs to be well-controlled to prevent silencing of ‘self’ genes. Here, we reveal a proteolytic mechanism that controls endogenous small interfering (22G) RNA activity in the Caenorhabditis elegans germline to protect genome integrity and maintain fertility. We find that WAGO-1 and WAGO-3 Argonaute proteins are produced as pro-proteins that are matured through proteolytic processing of their unusually proline-rich N-termini. In the absence of DPF-3, a P-granule-localized N-terminal dipeptidase orthologous to mammalian DPP8/9, processing fails, causing a change of identity of 22G RNAs bound to these WAGO proteins. Accumulation of repeat- and transposon-derived transcripts, DNA damage and sterility ensue. We propose that DPF-3 acts as a licensing factor for 22G RNA activity.
|
| |
|
| Overall design |
Wild type worms.9 Replicates; dpf-3 null worms. 3 replicates; DPF-3 S784A mutant worms. 3 Replicates; mut-2(ne3370). 3 replicates; mut-7(ne4255). 3 replicates
|
| |
|
| Contributor(s) |
Grosshans H, Gudipatti RK, Gypas F |
| Citation(s) |
33852894 |
| Submission date |
Jun 03, 2020 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Foivos Gypas |
| E-mail(s) |
foivos.gypas@fmi.ch
|
| Organization name |
FMI
|
| Lab |
Grosshans
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
|
| Samples (21)
|
|
| This SubSeries is part of SuperSeries: |
| GSE151717 |
Protease-mediated processing of Argonaute proteins controls small RNA association |
|
| Relations |
| BioProject |
PRJNA637042 |
| SRA |
SRP265813 |
Less...
More...