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Series GSE15165 Query DataSets for GSE15165
Status Public on Mar 12, 2009
Title Expression data from Arabidopsis seed compartments at the mature green stage.
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary We collected mature green seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing mature green-stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire mature green stage seed.
 
Overall design Mature green stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).
 
Contributor(s) Stone SL, Pelletier J, Kirkbride RC, Belmonte MF, Fei J, Hashimoto MM, Cheng C, Golberg RB, Harada JJ
Citation(s) 23319655, 20385809
Submission date Mar 10, 2009
Last update date Aug 28, 2018
Contact name John J Harada
E-mail(s) jjharada@ucdavis.edu
Phone 530-752-0673
Organization name University of California, Davis
Department Plant Biology
Lab Harada
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (14)
GSM378733 Laser capture microdissected (LCM) embryo proper at the mature green stage, biological replicate 1
GSM378734 Laser capture microdissected (LCM) embryo proper at the mature green stage, biological replicate 2
GSM378735 Laser capture microdissected (LCM) micropylar endosperm at the mature green stage, biological replicate 1
This SubSeries is part of SuperSeries:
GSE12404 Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development
Relations
BioProject PRJNA114971

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15165_RAW.tar 27.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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