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| Status |
Public on Dec 22, 2021 |
| Title |
Transcriptional profile of Smad4-deficient CTLs is similar to TRM |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transforming growth factor β (TGFβ) is a morphogenic protein that augments antiviral immunity by altering the functional properties of pathogen-specific memory CD8 T cells. During infection, TGFβ inhibits formation of effector (TEFF) and circulating memory CD8 T cells, while encouraging tissue-resident memory CD8 T cells (TRM) to settle in peripheral tissues. SMAD proteins are signaling intermediates that are used by members of the TGF cytokine family to modify gene expression. Using RNA-sequencing we determined that SMAD4 altered the transcriptional profile of antiviral CTLs during respiratory infection. Our data show that SMAD4 and TGFβ use alternate signaling pathways to cooperatively regulate a collection of genes that determine whether pathogen-specific memory CD8 T cells localize in peripheral or lymphoid tissues. During infection, SMAD4 acts independently of TGF to inhibit TRM development, while inducing genes that support formation of circulating memory CD8 T cells. The genes that are modulated by SMAD4 include several homing receptors (CD103, KLRG1 and CD62L) and transcription factors (Hobit and EOMES) that support memory formation.
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| Overall design |
mRNA expression analysis of 2 samples: WT & Smad4 deficient Early Effector CD8 T cells. D6 X31 infection Early effector subset (KLRG1lo, CD127lo, CD103lo)
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| |
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| Contributor(s) |
Hu Y, Chandiran K, Cauley LS |
| Citation(s) |
35942952 |
| |
| Submission date |
Jun 02, 2020 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Linda S Cauley |
| E-mail(s) |
lcauley@uchc.edu
|
| Organization name |
UConn Health
|
| Department |
Immunology
|
| Lab |
Cauley Lab
|
| Street address |
263 Farmington Ave
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA636744 |
| SRA |
SRP265603 |
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