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Series GSE15160 Query DataSets for GSE15160
Status Public on Mar 12, 2009
Title Expression data from Arabidopsis seed compartments at the heart stage.
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary We collected heart stage seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing heart stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire heart stage seed.
 
Overall design Heart stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 16 samples (embryo proper, micropylar endosperm, peripheral endosperm, seed coat and whole seeds, 2 biological replicates each; chalazal endosperm, chalazal seed coat, 3 biological replicates each).
 
Contributor(s) Stone SL, Pelletier J, Kirkbride RC, Belmonte MF, Fei J, Hashimoto MM, Cheng C, Golberg RB, Harada JJ
Citation(s) 23319655, 20385809
Submission date Mar 09, 2009
Last update date Aug 28, 2018
Contact name John J Harada
E-mail(s) jjharada@ucdavis.edu
Phone 530-752-0673
Organization name University of California, Davis
Department Plant Biology
Lab Harada
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (16)
GSM378645 Laser capture microdissected (LCM) embryo proper at the heart stage, biological replicate 1
GSM378646 Laser capture microdissected (LCM) embryo proper at the heart stage, biological replicate 2
GSM378647 Laser capture microdissected (LCM) micropylar endosperm at the heart stage, biological replicate 1
This SubSeries is part of SuperSeries:
GSE12404 Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development
Relations
BioProject PRJNA114933

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15160_RAW.tar 30.5 Mb (http)(custom) TAR (of CEL, CHP)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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