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Series GSE15159 Query DataSets for GSE15159
Status Public on Oct 19, 2009
Title Transcriptomic response of young adult N2, xpa1, and glp1 nematodes 3h after exposure to 50 J/m2 UVC radiation at 25°C
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary We compared the transcriptomic response to UVC radiation in widltype (N2), DNA repair-deficient (xpa-1), and germ cell-deficient (glp-1) young adult nematodes. We were interested in the wildtype response to this stressor, and postulated that the early (3h post-exposure) difference might be different in nucleotide excision repair-deficient (xpa-1) nematodes. We also hypothesized that the response would be different in young adults composed entirely of somatic cells (glp-1 strain at this temperature), compared to germ cell-bearing and gravid young adults (N2 and xpa-1). Finally, we compared constitutive differences in gene expression between the strains.
Overall design Young adult (24 h post-vulval crescent L4 stage) N2, xpa-1, and glp-1 nematodes were reared at 25°C and exposed to 50 J/m2 UVC radiation on K-agar plates without food as described (Meyer et al., 2007 Genome Biology 8(5):R70), placed on OP50-seeded plates for 2 h 45 minutes, washed off plates and rinsed with K-medium 3 times (~5 minutes each) by allowing settling, resuspending, settling again, etc. Nematodes were frozen and RNA extracted. The experiment was performed twice, once in 2007 and once in 2008, with 2 replicates each time, for a total “n” of 4 per strain per treatment (control vs UVC-exposed).
Contributor(s) Meyer JN, Andrews DM, Lehmann DW, Van Houten B
Citation(s) 19879883
Submission date Mar 09, 2009
Last update date Sep 25, 2019
Contact name NIEHS Microarray Core
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (24)
GSM378577 glp-1_UV_2008_rep1
GSM378578 glp-1_UV_2008_rep2
GSM378579 N2_control_2007_rep2
BioProject PRJNA114931

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Supplementary file Size Download File type/resource
GSE15159_RAW.tar 51.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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