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Series GSE15159 Query DataSets for GSE15159
Status Public on Oct 19, 2009
Title Transcriptomic response of young adult N2, xpa1, and glp1 nematodes 3h after exposure to 50 J/m2 UVC radiation at 25°C
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary We compared the transcriptomic response to UVC radiation in widltype (N2), DNA repair-deficient (xpa-1), and germ cell-deficient (glp-1) young adult nematodes. We were interested in the wildtype response to this stressor, and postulated that the early (3h post-exposure) difference might be different in nucleotide excision repair-deficient (xpa-1) nematodes. We also hypothesized that the response would be different in young adults composed entirely of somatic cells (glp-1 strain at this temperature), compared to germ cell-bearing and gravid young adults (N2 and xpa-1). Finally, we compared constitutive differences in gene expression between the strains.
 
Overall design Young adult (24 h post-vulval crescent L4 stage) N2, xpa-1, and glp-1 nematodes were reared at 25°C and exposed to 50 J/m2 UVC radiation on K-agar plates without food as described (Meyer et al., 2007 Genome Biology 8(5):R70), placed on OP50-seeded plates for 2 h 45 minutes, washed off plates and rinsed with K-medium 3 times (~5 minutes each) by allowing settling, resuspending, settling again, etc. Nematodes were frozen and RNA extracted. The experiment was performed twice, once in 2007 and once in 2008, with 2 replicates each time, for a total “n” of 4 per strain per treatment (control vs UVC-exposed).
 
Contributor(s) Meyer JN, Andrews DM, Lehmann DW, Van Houten B
Citation(s) 19879883
Submission date Mar 09, 2009
Last update date Sep 25, 2019
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (24)
GSM378577 glp-1_UV_2008_rep1
GSM378578 glp-1_UV_2008_rep2
GSM378579 N2_control_2007_rep2
Relations
BioProject PRJNA114931

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Supplementary file Size Download File type/resource
GSE15159_RAW.tar 51.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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