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| Status |
Public on Jul 23, 2020 |
| Title |
Bulk-ATAC sequencing in RUNX-altered murine lung adenocarcinoma cell lines |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Here, we using CRISPR activation and knockout studies to assess the implication of dysregulation of RUNX transcription factors on chromatin accessibility using bulk ATAC-sequencing. Tumor cell lines were derived from primary Kras G12D; p53 mutant mice (KP model) after initiation of tumors with SPC-Cre, resulting in lung adenocarcinoma. Cell lines were then expanded and profiled using bulk ATAC-sequencing.
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| Overall design |
Primary tumor derived cell lines were modified using CRISPR knockout and activation. Bulk ATAC-sequencing was completed on these cell lines.
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| Contributor(s) |
LaFave LM, Kartha VK |
| Citation missing |
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| |
| Submission date |
May 28, 2020 |
| Last update date |
Jul 23, 2020 |
| Contact name |
Lindsay M LaFave |
| E-mail(s) |
lmlafave@mit.edu
|
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Dr. Tyler Jacks
|
| Street address |
77 Massachusetts Avenue; 76-453
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (81)
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| Relations |
| BioProject |
PRJNA635682 |
| SRA |
SRP265155 |
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