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| Status |
Public on Oct 25, 2023 |
| Title |
Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
NGS (paired-end RNA-seq) of mouse hematopoietic stem and progenitor cells from young (4-6 mo) and old (31-33 mo) mice.
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| Overall design |
Wild-type HSPCs were isolated from the hind limb long bones of 4- to 6-month-old and 31- to 33-month-old male mice. c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following manufacturer’s instructions. After overnight growth in serum-free medium (StemSpan SFEM, Stem Cell Technologies), supplemented with murine TPO (20 ng/ml, PeproTech), SCF (10 ng/ml, PeproTech), and the beta-Catenin agonist CHIR99021 (250 nM, Stemgent), HSPCs were harvested as cell pellets. Immediately after, RNA extraction was carried out with the RNeasy Plus Mini kit with genomic DNA Eliminator columns (Qiagen) in combination with on-column DNaseI digestion (Qiagen), according to the vendor’s protocol.
Biological duplicates of two young and two old sets of hematopoietic stem and progenitor sets were used.
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| Contributor(s) |
Catic A |
| Citation(s) |
38553595 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DK115454 |
Transcript decay regulates hematopoietic aging |
BAYLOR COLLEGE OF MEDICINE |
Andre Catic |
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| |
| Submission date |
May 24, 2020 |
| Last update date |
Apr 09, 2024 |
| Contact name |
Andre Catic |
| E-mail(s) |
catic@post.harvard.edu
|
| Organization name |
Baylor College of Medicine
|
| Street address |
One Baylor Plaza
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA634795 |
| SRA |
SRP263347 |
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