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| Status |
Public on Apr 27, 2021 |
| Title |
System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation [Polysome profiling] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we deciphered the functions of Dhx36 and related molecular mechanisms in muscle stem cells and muscle regeneration. We found Dhx36 was highly induced in activated muscle stem cells during regeneration induced by injury. Conditional inactivation of Dhx36 in mouse muscle stem cells caused significant impaired regeneration, which was due to the dramatically decreased cell proliferation. Dhx36 was a well-known RNA helicase for binding/unwinding DNA/RNA G-quadruplexes and it was dominantly expressed in cytoplasm of proliferating myoblast. To examine whether Dhx36 regulates translation process, we conducted polysome profiling followed by RNA-seq in WT and Dhx36 KO myoblast cells.
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| |
|
| Overall design |
6 polysome profiling experiments performed.
|
| |
|
| Contributor(s) |
Wang H, Sun H, Chen X, Yuan J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 24, 2020 |
| Last update date |
May 01, 2021 |
| Contact name |
Jie Yuan |
| E-mail(s) |
jieyuan@link.cuhk.edu.hk
|
| Organization name |
The Chinese University of Hong Kong
|
| Department |
Orthopaedics and Traumatology
|
| Lab |
Wang Lab
|
| Street address |
Prince of Wales Hospital
|
| City |
HONG KONG |
| State/province |
HONG KONG |
| ZIP/Postal code |
NA |
| Country |
China |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE151124 |
System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation |
|
| Relations |
| BioProject |
PRJNA634791 |
| SRA |
SRP263310 |
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