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| Status |
Public on Feb 20, 2021 |
| Title |
eCLIP of Drosophila Asterix/Gtsf1 transfected in Drosophila ovarian somatic sheath (OSS) cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
Asterix/Gtsf1 has been previously been shown to be involved in the silencing of retrotransposons in animal germ cells through the piRNA pathway. Using eCLIP and a custom informatic pipeline, we found that Asterix/Gtsf1 binds directly to RNAs and preferentially interacts with tRNAs as a class. Given the role of tRNAs as primers for Long Terminal Repeat (LTR) transposons, this work implicates Asterix/Gtsf1 as exploiting tRNA dependence to identify transposon transcripts and promote piRNA silencing.
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| Overall design |
eCLIP of Drosophila Asterix/Gtsf1 transfected in Drosophila ovarian somatic sheath (OSS) cells. The protein was expressed with a C-terminal TEV-Strep tag from the vector pAWG under the control of the act5c promoter.
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| Contributor(s) |
Ipsaro JJ, Joshua-Tor L |
| Citation(s) |
33789107 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F32 GM097888 |
Structural Basis for RNA-induced Silencing via the Piwi Clade of Argonautes |
COLD SPRING HARBOR LABORATORY |
JONATHAN JAMES IPSARO |
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| |
| Submission date |
May 24, 2020 |
| Last update date |
Apr 01, 2021 |
| Contact name |
Jonathan James Ipsaro |
| E-mail(s) |
jipsaro@cshl.edu
|
| Organization name |
Cold Spring Harbor Laboratory
|
| Lab |
Leemor Joshua-Tor
|
| Street address |
1 Bungtown Road
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (6)
|
| GSM4566708 |
Asterix-Strep eCLIP - non-crosslinked_input |
| GSM4566709 |
Asterix-Strep eCLIP - crosslinked_input1 |
| GSM4566710 |
Asterix-Strep eCLIP - crosslinked_input2 |
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| This SubSeries is part of SuperSeries: |
| GSE151112 |
Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons |
|
| Relations |
| BioProject |
PRJNA634750 |
| SRA |
SRP263175 |
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