|
| Status |
Public on Mar 05, 2009 |
| Title |
H1 hESC Exposed to Butyrate |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To assess the effect of sodium butyrate exposure on human ESC grown without culture support for self-renewal (I.e. without conditioned medium and added bFGF) - three groups were compared - H1 culture in feeder conditioned medium vs without conditioned medium in 0.2 mM sodium butyrate vs. grown in sodium butyrate for 4 passages followed by return to conditioned medium conditions for 3 passages. The three groups were grown in triplicate and compared on Agilent whole human genome array
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| Overall design |
H1 hESC were grown under 3 conditions: A. 48 passages total, the last 9 passages off of feeders on Matrigel in feeder conditioned medium B. 48 passages total, within the last 9 passges, #39-41 were in conditioned medium and #42-48 were without conditioned medium and added bFGF but with 0.2 mM sodium butyrate on Matrigel C. 49 passages total, within the last 10 passages #39-41 were in conditioned medium, #42-46 in 0.2 mM sodium butyrate (without feeder of bFGF support) and #47-49 back to conditioned medium + bFGF. The RNA from these cells were compared via Agilent whole human genome array.
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| |
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| Contributor(s) |
Ware C, Balu A, Mecham B |
| Citation(s) |
19341625 |
| |
| Submission date |
Mar 04, 2009 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Brig Mecham |
| E-mail(s) |
bmecham@fhcrc.org
|
| Organization name |
University of Washington
|
| Street address |
1100 Fairview Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1708 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA123103 |
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