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Series GSE150815 Query DataSets for GSE150815
Status Public on Aug 31, 2020
Title Mycobacterium tuberculosis small RNA MTS1338 is required for bacterial virulence and regulates the expression of an immune-evasion molecules
Organism Mycolicibacterium smegmatis
Experiment type Expression profiling by high throughput sequencing
Summary Bacterial small non-coding RNAs play an essential role in adaptation to stresses. M. tuberculosis small RNA MTS1338 was shown to contribute to successful persistence of mycobacteria within host cells. In this study, we expressed MTS1338 in Mycobacterium smegmatis, and studied transcriptomic and proteomic changes in the recombinant strain. We found that MTS1338 expression resulted to DosR regulon activation, metabolic changes characteristic for hypoxia, mycolic acids synthesis, as well as rearrangement of iron metabolism. These metabolic changes slow down bacterial growth in vitro, provide an increase of non-pathogenic M.smegmatis survival in the infected THP-1macrophages, and also influence phagosome maturation.
 
Overall design THP-1 Macrophages were infected with two strains of M. smegmatis. One strain contaned a vector that expresses M.tuberculosis small non-coding RNA MTS1338 (pMV_MTS1338) and other strain was a control and contained an empty vector (pMV_Empty). In each group, three independent biological samples were sequenced.
 
Contributor(s) Grigorov A, Salina EG, Bychenko O, Skvortsova Y, Azhikina T
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Submission date May 19, 2020
Last update date Sep 02, 2020
Contact name Artem Grigorov
E-mail(s) artgrigorov@gmail.com
Organization name Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Department Genomics and Postgenomic Technologies
Lab Regulatory Transcriptomics
Street address Ulitsa Miklukho-Maklaya, 16/10
City Moscow
ZIP/Postal code 117997
Country Russia
 
Platforms (1)
GPL28556 Illumina NovaSeq 6000 (Mycolicibacterium smegmatis)
Samples (6)
GSM4559147 msm pMV_Empty rep1
GSM4559148 msm pMV_Empty rep2
GSM4559149 msm pMV_Empty rep3
Relations
BioProject PRJNA633785
SRA SRP262279

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150815_Counts_all.csv.gz 93.3 Kb (ftp)(http) CSV
GSE150815_DifferentialExpression.csv.gz 236.3 Kb (ftp)(http) CSV
GSE150815_RAW.tar 190.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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