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Series GSE150657 Query DataSets for GSE150657
Status Public on Sep 14, 2021
Title Lung epithelial signaling mediates early vaccine-induced CD4+ T cell activation and Mtb control
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Other
Summary Tuberculosis (TB) is the leading cause of death due to a single infectious agent. The development of a TB vaccine that induces durable and effective immunity to Mycobacterium tuberculosis (Mtb) infection is urgently needed. Complete and early Mtb control can be induced in M. bovis Bacillus Calmette–Guérin (BCG) vaccinated hosts when the innate immune response is targeted to generate effective vaccine-induced immunity. In the present study, we show that the superior and early Mtb control that is mediated by innate activation of DCs is associated with mucosal localization of clonal activated vaccine-induced CD4+ T cells in the lung. Our studies also show that the lung epithelial cell signaling through the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB pathway is critical for the mucosal localization of activated vaccine-induced CD4+ T cells for rapid Mtb control. Thus, our study provides novel insights into the immune mechanisms that can overcome TB vaccine bottlenecks and provide early rapid Mtb control.
 
Overall design Lung cells were isolated from Mtb-infected unvaccinated mice (Unvac), Mtb-infected BCG vaccinated mice (Vac), and Mtb-infected BCG vaccinated mice that received Z-DC transfer (Vac+Z-DC) and subjected to single cell RNA sequencing (scRNA-Seq) to define immune cell populations driving enhanced vaccine-induced immunity.
 
Contributor(s) Das S, Marin-Agudelo N, Esaulova E, Ahmed M, Swain A, Rosa BA, Mitreva M, Rangel-Moreno J, Kaushal D, Artyomov MN, Khader SA
Citation(s) 34253059
Submission date May 15, 2020
Last update date Sep 15, 2021
Contact name Maxim N. Artyomov
E-mail(s) martyomov@pathology.wustl.edu
Organization name Washington University in St.Louis
Department Immunology&Pathology
Street address 660 S. Euclid Avenue, Campus Box 8118
City St.Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (7)
GSM4555847 Mtb_unvaccinated_1
GSM4555848 Mtb_unvaccinated_2
GSM4555849 Mtb_bcg_1
Relations
BioProject PRJNA633109
SRA SRP261839

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150657_RAW.tar 147.2 Mb (http)(custom) TAR (of H5, JSON)
GSE150657_TCR_by_cell.txt.gz 1.2 Mb (ftp)(http) TXT
GSE150657_TCR_summary.txt.gz 327.5 Kb (ftp)(http) TXT
GSE150657_T_cells_expData.Rda.gz 92.5 Mb (ftp)(http) RDA
GSE150657_T_cells_meta_data.tsv.gz 1.1 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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