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| Status |
Public on May 15, 2020 |
| Title |
DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
Third-party reanalysis
|
| Summary |
Duplication of mammalian genomes requires replisomes to overcome numerous impediments during passage through open (eu) and condensed (hetero) chromatin. Typically, studies of replication stress characterize mixed populations of challenged and unchallenged replication forks, averaged across S phase, and model a single species of “stressed” replisome. However, in cells containing potent obstacles to replication, we find two different lesion proximal replisomes. One is bound by the DONSON protein and is more frequent in early S phase, in regions marked by euchromatin. The other interacts with the FANCM DNA translocase, is more prominent in late S phase, and favors heterochromatin. The two forms can also be detected in unstressed cells. CHIP-seq of DNA associated with DONSON or FANCM confirms the bias of the former towards regions that replicate early and the skew of the latter towards regions that replicate late
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| Overall design |
FANCM and GFP(-DONSON) Chip-seq analysis in asynchronous Hela cells stably expressing GFP-DOSNON
Third-party reanalysis: Hela_eigenvector_HiC.hg38.bedgraph was calculated on Hi-C data for Hela cells downloaded from NCBI, dbGaP phs000640.v8.p1. See README files for data processing details.
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| Contributor(s) |
Bellani M, Zhang J, Seidman M |
| Citation(s) |
32769987 |
| |
| Submission date |
May 14, 2020 |
| Last update date |
Aug 24, 2020 |
| Contact name |
Marina A Bellani |
| E-mail(s) |
marinab@nih.gov
|
| Organization name |
NIA
|
| Department |
LMB
|
| Lab |
Gene Targeting Section
|
| Street address |
251 Bayview Blvd
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA632751 |
| SRA |
SRP261582 |
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