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| Status |
Public on Mar 07, 2009 |
| Title |
Gibberellin-induced gene expression in maize mesocotyl under deep-sowing condition |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
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| Summary |
In order to elucidate the molecular mechanism of gibberellin (GA)-induced mesocotyl elongation, gene expression profiling analyses were performed in a deep-sowing tolerant maize inbred line 3681-4. Gene expression studies combing Affymetrix GeneChip analysis and Real-time PCR were employed to determine the molecular mechanism underlying GA promotion of maize mesocotyl elongation. These studies showed that the GA receptor GID1, the transcriptional factor MYB, and the genes encoding DELLA protein DWRF8, kinases, Raf, LRR, RLCK, and involved in flavonoid biosynthesis, aminosugars metabolism, cell wall synthesis and modification, might play critical roles in maize mesocotyl elongation.
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| Overall design |
In two independent experiments, we generate 10-day-old maize mesocotyl-specific gene expression profiles through comparing genome-wide expression patterns of GA3 treatment and UCZ (a GA biosynthesis inhibitor) treatment under 20 cm deep-sowing condition in darkness by using 17,555 Affymetrix maize whole genome array.
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| Contributor(s) |
Guangwu Z, Junjie F, Guoying W, Pan M, Liwen W, Jianhua W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 28, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Guoying Wang |
| E-mail(s) |
gywang@caas.net.cn
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| Phone |
86-10-62162927
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Department |
Institute of Crop Sciences
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| Lab |
Maize Molecular Biology
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| Street address |
Zhongguancun Nandajie 12
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| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL4032 |
[Maize] Affymetrix Maize Genome Array |
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| Samples (6)
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| GSM375788 |
Deep-sowing 10d treatment Control, biological rep1 |
| GSM375791 |
Deep-sowing 10d treatment GA3, biological rep1 |
| GSM375794 |
Deep-sowing 10d treatment UCZ, biological rep1 |
| GSM375795 |
Deep-sowing 10d treatment Control, biological rep2 |
| GSM375796 |
Deep-sowing 10d treatment GA3, biological rep2 |
| GSM375797 |
Deep-sowing 10d treatment UCZ, biological rep2 |
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| Relations |
| BioProject |
PRJNA114815 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15048_RAW.tar |
13.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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