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| Status |
Public on May 21, 2020 |
| Title |
Genome-wide DNA methylation analysis in cfDNA from pancreatic cancer patients |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Heterogeneity in DNA methylation status may exist between primary tumors and cfDNA. We compared the genome-wide DNA methylation status between FNA sample of pancreatic tumor and cfDNA samples from the same patients. MBD-sequencing (seq) was performed in paired samples of FNA and cfDNA from two patients.
The majority of DNA methylation peaks overlapped in FNA and cfDNA samples (82.6% and 82.9% in patients #1 and #73, respectively). DNA methylation levels of each gene in FNA and cfDNA samples were highly correlated with each other (r=0.98 and 0.97 in samples 1 and 73, respectively)
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| |
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| Overall design |
DNA methylation status was analyzed by MBD-seq in cfDNA from pancreatic cancer patients and corresponding tumor and cfDNA from normal patients.
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| |
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| Contributor(s) |
Kondo Y, Shinjo K |
| Citation(s) |
32520974 |
| |
| Submission date |
May 13, 2020 |
| Last update date |
Aug 20, 2020 |
| Contact name |
Keiko Shinjo |
| E-mail(s) |
kshinjo@med.nagoya-u.ac.jp
|
| Phone |
0527442463
|
| Organization name |
Nagoya Univ. Graduate School of Medicine
|
| Department |
Cancer Biology
|
| Street address |
65 Tsurumai, Showa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA632511 |
| SRA |
SRP261413 |
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