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Series GSE15038 Query DataSets for GSE15038
Status Public on Feb 27, 2009
Title Identification of novel transcriptional regulators involved in differentiation and function of monocyte and macropahge
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Maturation of blood monocytes into tissue macrophages and subsequent inflammatory response was mimicked in U937 cells of human histocytic lymphoma origin. Whole genome array analysis was employed to evaluate gene expression profile to identify underlying transcriptional networks implicated during the processes of differentiation and inflammation. In addition to already known transcription factors (i.e. MAFB, EGR, IRF, BCL6, NFkB, AP1, Nur77), gene expression analysis further revealed novel genes (i.e. MEF2, BRI, HLX, HDAC5, H2AV, TCF7L2, NFIL3) previously uncharacterized to be involved in the differentiation process. A total of 58 selected genes representing cytokines, chemokines, surface antigens, signaling molecules and transcription factors were validated by real time PCR and compared to primary monocyte-derived macrophages. Beside the verification of several new genes, the comparison reveals individual heterogeneity of blood donors.
Overall design U937 cells were differentiated into monocytes by VitD3 (24 hrs) or into marophages by PMA (6 hrs, 12 hrs, 24 hrs and 32 hrs). Cells differentiated for 24 hrs by PMA are further stimulated by LPS for 2 hrs. Total RNA from 6 case samples were subjected to differential gene expression in comparison to undifferentiated U937 cells.
Contributor(s) Baek S, Haas S, Hackstein H, Bein G, Hernandez-Santana M, Sauer S, Lehrach H, Seitz H
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Submission date Feb 27, 2009
Last update date Mar 21, 2012
Contact name Agnes Baek
Organization name Max Planck Institute for Molecular Genetics
Department Dept. of Vetebrate Genomics
Street address Ihnestrasse 63-73
City Berlin
ZIP/Postal code 14195
Country Germany
Platforms (1)
GPL5104 Sentrix HumanRef-8 v2 Expression BeadChip
Samples (7)
GSM375668 U937 cells_undifferentiated
GSM375669 U937 cells_PMA_6hrs
GSM375670 U937 cells_PMA_12hrs
BioProject PRJNA114803

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Supplementary file Size Download File type/resource
GSE15038.txt.gz 1.5 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data provided as supplementary file
Raw data is available on Series record

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