|
| Status |
Public on Jan 04, 2021 |
| Title |
Comparative genome-wide transcriptional profile after RTEL1 depletion and G4 stabilisation. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to compare the transcription profile of mouse embryonic fibroblasts upon deletion of the helicase RTEL1 and stabilisation of G-quadruplexes (G4) with the G4 stabilisation drug TMPyP4.
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| |
|
| Overall design |
RNA-seq analysis of Rtel1f/f Mouse Embryonic Fibroblasts cells, infected with Ad-GFP or Ad-Cre adenovirus. RNA was collected 96 hours after the infection. In the indicated cases, cells were treated with TMPyP4 for 48 hours prior to the RNA collection. Three conditions were compared: cells infected with Ad-GFP, cells infected with Ad-Cre, and cells infected with Ad-GFP and treated with TMPyP4.
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| |
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| Contributor(s) |
Margalef P, Kotsantis P, Segura-Bayona S, Poetsch AR, Patel H, Ruis P, Marzec P, Bellelli R, Hewitt G, Stanage TH, Goldstone R, Boulton SJ |
| Citation(s) |
33357438 |
| |
| Submission date |
May 04, 2020 |
| Last update date |
Jan 04, 2021 |
| Contact name |
Simon J Boulton |
| E-mail(s) |
simon.boulton@crick.ac.uk
|
| Organization name |
The Francis Crick Institute
|
| Lab |
DSB Repair Metabolism Lab
|
| Street address |
1 Midland Rd
|
| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (21)
|
| GSM4514214 |
Rtel1f/f Ad-Cre RTEL1 CA biological replicate 1 |
| GSM4514215 |
Rtel1f/f Ad-Cre RTEL1 CA biological replicate 2 |
| GSM4514216 |
Rtel1f/f Ad-Cre RTEL1 CA biological replicate 3 |
| GSM4514217 |
Rtel1f/f Ad-GFP RTEL1 CA biological replicate 1 |
| GSM4514218 |
Rtel1f/f Ad-GFP RTEL1 CA biological replicate 2 |
| GSM4514219 |
Rtel1f/f Ad-GFP RTEL1 CA biological replicate 3 |
| GSM4514220 |
Rtel1f/f Ad-Cre biological replicate 1 |
| GSM4514221 |
Rtel1f/f Ad-Cre biological replicate 2 |
| GSM4514222 |
Rtel1f/f Ad-Cre biological replicate 3 |
| GSM4514223 |
Rtel1f/f Ad-GFP biological replicate 1 |
| GSM4514224 |
Rtel1f/f Ad-GFP biological replicate 2 |
| GSM4514225 |
Rtel1f/f Ad-GFP biological replicate 3 |
| GSM4514226 |
Rtel1f/f Ad-GFP TMPyP4 biological replicate 1 |
| GSM4514227 |
Rtel1f/f Ad-GFP TMPyP4 biological replicate 2 |
| GSM4514228 |
Rtel1f/f Ad-GFP TMPyP4 biological replicate 3 |
| GSM4514229 |
Trf1f/f Ad-Cre biological replicate 1 |
| GSM4514230 |
Trf1f/f Ad-Cre biological replicate 2 |
| GSM4514231 |
Trf1f/f Ad-Cre biological replicate 3 |
| GSM4514232 |
Trf1f/f Ad-GFP biological replicate 1 |
| GSM4514233 |
Trf1f/f Ad-GFP biological replicate 2 |
| GSM4514234 |
Trf1f/f Ad-GFP biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE161597 |
RTEL1 regulates G4/R-loops to avert replication-transcription collisions |
|
| Relations |
| BioProject |
PRJNA630270 |
| SRA |
SRP260044 |
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