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Series GSE14899 Query DataSets for GSE14899
Status Public on Nov 01, 2009
Title A microRNA microarray analysis of C.elegans Parkinsons disease models
Organism Caenorhabditis elegans
Experiment type Non-coding RNA profiling by array
Summary MicroRNAs (miRNAs) play an important role in human brain development and maintenance. To search for miRNAs potentially involved with molecular mechanisms in the pathogenesis of Parkinsons disease (PD), we utilized miRNA microarrays to identify expression changes of 115 annotated Caenorhabditis elegans (C.elegans) miRNAs in human α-synuclein A53T transgenic, dopamine deficient catecholamine transporter gene cat-1 mutant and parkin gene pdr-1 mutant C.elegans strains. Twelve miRNAs were found regulated differentially in α-synuclein transgenic C. elegans, five in cat-1 mutants and three in pdr-1 mutants. The family of miR64/65 appeared co-under-expressed in α-synuclein and cat-1 strains and members of let-7 family co-under-expressed (except miR-241 over-expressed) in a-synuclein and pdr-1 strains. Class H serpentine receptor (srh) family of G-protein-coupled receptor genes were computationally identified to be highly over-represented target candidates for regulated miRNAs.These results indicate that miRNAs are misregulated in C. elegans PD models and suggest a role for these molecules in the disease pathogenesis.
 
Overall design Two color NCode microRNA microrarrays were used (Invitrogen) to measure miRNA expression changes in human alpha-synuclein trangenic, cat-1 mutant, and pdr-1 mutant C.elegans strains. The experiment contains four independent biological replicates (worm populations)/strain. Strain N2 was used as reference channel, also prepared from four independently isolated small RNA samples.
 
Contributor(s) Asikainen S, Louhiranta K, Rudgalvyte M, Lakso M, Nass R, Wong G
Citation(s) 20091141
Submission date Feb 19, 2009
Last update date Mar 20, 2012
Contact name Suvi Tuulia Asikainen
E-mail(s) suvi.asikainen@helsinki.fi
Phone +358503311688
Organization name Karolinska Institutet
Department CLINTEC
Lab Outi Hovatta's lab
Street address Karolinska University Hospital
City Stockholm
State/province Huddinge
ZIP/Postal code 14186
Country Sweden
 
Platforms (1)
GPL8200 NCode Caenorhabditis elegans miRNA Microarray Kit V2
Samples (12)
GSM372189 alpha-synuclein transgenic strain vs N2, replicate 1
GSM372190 alpha-synuclein transgenic strain vs N2, replicate 2
GSM372191 alpha-synuclein transgenic strain vs N2, replicate 3
Relations
BioProject PRJNA111999

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14899_RAW.tar 350.0 Kb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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