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| Status |
Public on Aug 25, 2020 |
| Title |
A single cell atlas of the human proximal epididymis reveals cell-type specific functions and distinct roles for CFTR |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Spermatozoa released from the testis are unable to fertilize an egg without a coordinated process of maturation in the lumen of the epididymis. Relatively little is known about the molecular events that integrate this critical progression along the male genital ducts in man. Here we use single cell RNA-sequencing to construct an atlas of the human proximal epididymis. We find that the cystic fibrosis transmembrane conductance regulator (CFTR), which is pivotal in normal epididymis fluid transport, is most abundant in surface epithelial cells in the efferent ducts and in rare clear cells in the caput epididymis, suggesting region-specific functional properties. We reveal transcriptional signatures for multiple cell clusters, which identify the individual roles of principal, apical, narrow, basal, clear, halo and stromal cells in the epididymis. A marked cell-type-specific distribution of function is seen along the duct with local specialization of individual cell types integrating processes of sperm maturation.
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| Overall design |
using single-cell RNA sequencing to map cell-type-specific gene expression in the HEE tissues
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| Contributor(s) |
Leir S, Yin S, Kerschner JL, Harris A |
| Citation(s) |
32855272 |
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| Submission date |
Apr 20, 2020 |
| Last update date |
Nov 24, 2020 |
| Contact name |
Ann Harris |
| E-mail(s) |
axh575@case.edu
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| Organization name |
Case Western Reserve Uni
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| Street address |
10900 Euclid Ave
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| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (3) |
| GSM4486714 |
single cell RNA seq of human epididymis epithelial tissue [AXH009] |
| GSM4486715 |
single cell RNA seq of human epididymis epithelial tissue [AXH012] |
| GSM4486716 |
single cell RNA seq of human epididymis epithelial tissue [AXH014] |
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| Relations |
| BioProject |
PRJNA626628 |
| SRA |
SRP257542 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE148963_RAW.tar |
70.3 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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