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| Status |
Public on May 31, 2020 |
| Title |
microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Extracted total RNA from FFPE tissue were used for RNA labeling and hybridization on to the Agilent Human SurePrint 8 × 60k v16 miRNA microarray chip according to the manufacturer's protocol.
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| Overall design |
Total RNA was extracted from FFPE tissue using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol. RNA was then subjected to labeling and hybridization on to the Agilent Human SurePrint 8×60k v16 miRNA microarray chip according to the manufacturer's protocol.
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| |
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| Contributor(s) |
Alajez NM |
| Citation(s) |
32509578 |
| |
| Submission date |
Apr 17, 2020 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Nehad M Alajez |
| E-mail(s) |
nalajez@gmail.com
|
| Organization name |
King Saud University College of Medicine
|
| Department |
Anatomy
|
| Lab |
Stem Cell Unit
|
| Street address |
1st floor College of Medicne
|
| City |
Riyadh |
| ZIP/Postal code |
11461 |
| Country |
Saudi Arabia |
| |
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| Platforms (1) |
| GPL21576 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (Probe Name version) |
|
| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE148848 |
microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors |
|
| Relations |
| BioProject |
PRJNA625954 |
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