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Status |
Public on May 07, 2020 |
Title |
Global identification of RNase E sites in Vibrio cholerae |
Organism |
Vibrio cholerae |
Experiment type |
Expression profiling by high throughput sequencing Other
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Summary |
In this study, we employed TIER-seq in V. cholerae to identify cleavage sites of RNase E on a genome-wide scale.
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Overall design |
V. cholerae wild-type and rneTS (carrying the rne-3071 allele encoding a temperature-sensitive variant of RNase E) were grown in triplicates at 30°C to late exponential phase, divided in half and grown at 30°C or 44°C for 1h before collection of RNA samples. Following RNA-seq, 5'ends profiles were compared to detect RNase E sites (5' ends missing in the rneTS mutant strain at 44°C compared to the wild-type).
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Contributor(s) |
Hoyos M, Huber M, Förstner KU, Papenfort K |
Citation missing |
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Submission date |
Apr 14, 2020 |
Last update date |
May 09, 2020 |
Contact name |
Konrad U. Förstner |
E-mail(s) |
foerstner@zbmed.de
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Organization name |
ZB MED - Information Centre for Life Sciences
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Department |
Information Services
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Lab |
Förstner Lab
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Street address |
Gleueler Str. 60
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City |
Cologne |
State/province |
North Rhine-Westphalia |
ZIP/Postal code |
50931 |
Country |
Germany |
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Platforms (1) |
GPL25005 |
Illumina NextSeq 500 (Vibrio cholerae) |
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Samples (12)
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Relations |
BioProject |
PRJNA625325 |
SRA |
SRP256380 |