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| Status |
Public on May 07, 2020 |
| Title |
Global identification of RNase E sites in Vibrio cholerae |
| Organism |
Vibrio cholerae |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
In this study, we employed TIER-seq in V. cholerae to identify cleavage sites of RNase E on a genome-wide scale.
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| Overall design |
V. cholerae wild-type and rneTS (carrying the rne-3071 allele encoding a temperature-sensitive variant of RNase E) were grown in triplicates at 30°C to late exponential phase, divided in half and grown at 30°C or 44°C for 1h before collection of RNA samples. Following RNA-seq, 5'ends profiles were compared to detect RNase E sites (5' ends missing in the rneTS mutant strain at 44°C compared to the wild-type).
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| Contributor(s) |
Hoyos M, Huber M, Förstner KU, Papenfort K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 14, 2020 |
| Last update date |
May 09, 2020 |
| Contact name |
Konrad U. Förstner |
| E-mail(s) |
foerstner@zbmed.de
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| Organization name |
ZB MED - Information Centre for Life Sciences
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| Department |
Information Services
|
| Lab |
Förstner Lab
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| Street address |
Gleueler Str. 60
|
| City |
Cologne |
| State/province |
North Rhine-Westphalia |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL25005 |
Illumina NextSeq 500 (Vibrio cholerae) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA625325 |
| SRA |
SRP256380 |
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